Zhang Yangpei, Bai Xiaobin, Chen Yueneng, et al. INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION[J]. Journal of Radiation Research and Radiation Processing, 1989,7(4):25-29.
Zhang Yangpei, Bai Xiaobin, Chen Yueneng, et al. INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION[J]. Journal of Radiation Research and Radiation Processing, 1989,7(4):25-29.DOI:
The DNA-mediated gene transfer had been carried out by both Calcium phosphate coprecipitation and electroporation method. The cellular DNA and DNA fragments from human cervical carcinoma HeLaS3 cells were introduced with PSV2Neo DNA into XP20S (SV40) cells. The transfectants were picked up after twice selections by G418 and 3J/m,2, UV-irradiation. The results showed that cellular DNA and Bgl Ⅰ, Xho Ⅰ digested DNA fragments from HeLaS 3 could correct the deficiency of excision repair gene in XP cells, and cause the recipient cells resistant to UV irradiation. The second transfection experiment confirmed that HeLaS3 DNA were really integrated into XP cell chromosome and stably expressed within the cell genome.
关键词
紫外辐射切除修复基因转移XP细胞HeLaS 3 DNA
Keywords
UV-irradiationExcision repairGene transferXP cellHeLaS3 DNA