Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice

XUE Yizhe; ZHANG Zhaowen; GUO Ling; LAI Panpan; LIN Jiajin; QIN Tongzhou; JING Yuntao; WANG Xing; ZHOU Guiqiang; DING Guirong

doi:10.11889/j.1000-3436.2022-0062

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Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2023-09-15

- Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice
- Journal of Radiation Research and Radiation Processing Vol. 41, Issue 1, Pages: 50-59(2023)
- 作者机构：
  
  1.空军军医大学军事预防医学系辐射防护医学教研室西安 710032
- 作者简介：
  
  XUE Yizhe （female） was born in March 1991, and obtained her master's degree from Shaanxi Normal University in June 2017
  DING Guirong, doctoral degree, professor, E-mail: dingzhao@fmmu.edu.cn
- 基金信息：
  
  Special Project of Air Force Medical University(2021HKYX08)
- DOI：10.11889/j.1000-3436.2022-0062
  CLC：
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XUE Yizhe, ZHANG Zhaowen, GUO Ling, et al. Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice. [J]. Journal of Radiation Research and Radiation Processing 41(1):50-59(2023)
DOI：

XUE Yizhe, ZHANG Zhaowen, GUO Ling, et al. Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice. [J]. Journal of Radiation Research and Radiation Processing 41(1):50-59(2023) DOI： 10.11889/j.1000-3436.2022-0062.

摘要

为探讨5G手机射频辐射（Radiofrequency radiation，RFR）对成年雄性小鼠精子质量和性激素水平的影响，24只健康成年雄性C57BL/6小鼠随机分为假暴露组（Sham）和4.9 GHz暴露组（4.9 GHz RFR），每组12只。暴露组小鼠接受功率密度50 W/m,2,、频率为4.9 GHz的RFR全身暴露，每天1 h，连续暴露21 d。暴露结束后，通过检测精子数量和畸形率评估精子质量；通过苏木精-伊红（HE）染色观察睾丸组织形态；酶联免疫吸附试验（ELISA）测定睾酮（T）、卵泡刺激素（FSH）、黄体生成素（LH）、促性腺激素释放激素（GnRH）、胶质源性神经营养因子（GDNF）和干细胞因子（SCF）的水平；蛋白免疫印迹（Western blot）检测睾丸组织中GDNF和SCF以及血睾屏障紧密连接相关蛋白（ZO-1和Occludin）的水平。与Sham组相比，4.9 GHz组睾丸形态、精子数量和畸形率无显著改变；血清中FSH和LH含量无明显改变；睾丸内GDNF、SCF、ZO-1和Occludin蛋白水平均无明显变化；但是血清中T和GnRH含量显著降低。结果表明，本实验条件下，4.9 GHz RFR对小鼠精子质量无明显影响，但可导致性激素水平紊乱。

Abstract

To investigate the effects of 5G mobile phone radiofrequency radiation (RFR) on sperm quality and sex hormone level of adult male mice, 24 healthy adult male C57BL/6 mice were randomly divided into two groups, the sham-exposure group (Sham) and 4.9 GHz-exposure group (4.9 GHz RFR), with 12 mice in each group. The mice in the exposure group were exposed to RFR with a power density of 50 W/m,2, and frequency of 4.9 GHz for 21 consecutive days, for 1 h a day. After exposure, the sperm quality was evaluated by detecting the sperm quantity and abnormality rate; Histomorphology of the testis was determined using Hematoxylin Eosin (HE) staining; the levels of testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), gonadotropin releasing hormone (GnRH), glial derived neurotrophic factor (GDNF), and stem cell factor (SCF) were measured using enzyme-linked immunosorbent assay (ELISA); A western blot was used to detect the levels of GDNF, SCF and tight junction related proteins (ZO-1 and Occludin) of the blood-testis barrier (BTB) in testis tissue. Compared with the Sham group, no significant change in testicular morphology, sperm count, and sperm abnormality rate was observed in the 4.9 GHz RFR group; The content of FSH and LH in serum showed no significant differences; and the levels of GDNF, SCF, ZO-1 and Occludin in the testis showed no significant differences. However, the concentration of T and GnRH in serum decreased significantly. The results showed that under the experimental conditions, 4.9 GHz RFR had no significant effect on the sperm quality of mice, but could lead to changes in sex hormone levels.

关键词

手机射频辐射小鼠睾丸精子性激素

Keywords

Mobile phone radio frequency radiationMiceTestesSpermSex hormones

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