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YANG Bing SUN Yuanming TANG Quan ZHOU Hui ZHANG Xiaodong WANG Qin HAN Ying FAN Tiqiang FAN Feiyue

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 31, Issue 2, Pages: 2-020202(2013)
- 作者机构：
  
  1. 1（北京协和医学院和中国医学科学院放射医学研究所天津分子核医学重点实验室）,天津,300192
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YANG Bing SUN Yuanming TANG Quan ZHOU Hui ZHANG Xiaodong WANG Qin HAN Ying FAN Tiqiang FAN Feiyue. [J]. Journal of Radiation Research and Radiation Processing 31(2):2-020202(2013)
DOI：

YANG Bing SUN Yuanming TANG Quan ZHOU Hui ZHANG Xiaodong WANG Qin HAN Ying FAN Tiqiang FAN Feiyue. [J]. Journal of Radiation Research and Radiation Processing 31(2):2-020202(2013) DOI：

摘要

研究辐射对于Notch通路中重要的信号分子Notch1和Runx2，以及其对成骨细胞分化的影响。采用原代骨髓间充质干细胞诱导分化为成骨细胞，经过0、2和4 Gy 137Cs γ射线照射后，采用实时定量PCR以及Western Blot方法检测辐射对于Notch1、Runx2以及ALP mRNA和蛋白水平表达的影响。实验发现4 Gy照射会导致Notch1 mRNA（p<0.05）和蛋白表达上调。2 Gy和4 Gy照射均能引起Runx2 mRNA（p<0.05）和蛋白表达水平上调，并导致ALP mRNA（p<0.01）和蛋白水平表达的下调。研究表明：4 Gy照射Notch1表达增高，导致ALP表达水平降低，对成骨细胞的分化产生了抑制，并且该抑制作用并没有受到Runx2表达水平上调的影响。在辐射影响下Runx2并不能对Notch信号通路发生抑制作用，从而解除其对成骨细胞分化的抑制作用。

Abstract

To investigate the radiation effect on Notch pathway in osteoblast and osteoblast differentiation, the expression of Notch1 and Runx2 were analyzed. Osteoblast differentiated from Bone marrow stromal cells (BMSCs) was analyzed by real-time PCR and western blot after 2 Gy or 4 Gy 137Cs γ-irradiation. Notch1 mRNA (p<0.05) and protein expression level were up-regulated after 4 Gy irradiation. 2 Gy and 4Gy irradiation increased Runx2 mRNA (p<0.05) and protein expression level. ALP mRNA (p<0.01) and protein expression level were repressed by 2 Gy and 4 Gy irradiation. These results indicated that up-regulated Notch1 could inhibit osteoblast differentiation after irradiation of 4 Gy. Further more, up-regulated Runx2 expression did not repressed Notch signaling after irradiation of 4 Gy.Cited

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电离辐射成骨细胞Notch通路Notch1Runx2

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