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HE Binbin1; LI Binyuan2; MA Yun2; YANG Jie1; WANG Sanhu2; SU Zehong2; TANG Yan1; HE Shuya1; 2

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 31, Issue 2, Pages: 1-020201(2013)
- 作者机构：
  
  1. 1（南华大学公共卫生学院）,衡阳,421001
  2. 2（南华大学生物化学与分子生物学教研室）,衡阳,421001
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HE Binbin1, LI Binyuan2, MA Yun2, et al. [J]. Journal of Radiation Research and Radiation Processing 31(2):1-020201(2013)
DOI：

HE Binbin1, LI Binyuan2, MA Yun2, et al. [J]. Journal of Radiation Research and Radiation Processing 31(2):1-020201(2013) DOI：

摘要

克隆与辐射抗性相关但功能未知的新基因DR_2566，并应用生物信息学初步探讨其功能。应用PCR技术从耐辐射奇球菌基因组中扩增DR_2566基因全长ORF，选用pGEM-T载体进行TA克隆，通过限制性酶切及测序进行鉴定。应用生物信息学初步分析其染色体定位、蛋白序列、结构域及功能。成功克隆了耐辐射球菌未知功能基因DR_2566，生物信息学分析显示此基因全长为507 bp，编码168个氨基酸，其编码蛋白的理论分子量为19.136 KDa，三级结构预测显示DR_2566蛋白质具有短修复内切酶性质。成功克隆了耐辐射奇球菌基因DR_2566全长ORF，生物信息学预测DR_2566 蛋白可能具有错配修复作用。

Abstract

The objective of this study was to clone DR_2566 and to investigate its function preliminary using bio-informatics. Full-length open reading frame (ORF) of DR_2566 gene was amplified by PCR, and pGEM-T was chosen as a vector for gene TA cloning. After ligation, restriction endonuclease analysis and sequencing were carried out for identification. On the other hand, bio-informatics was used to analyze the localization of the target gene on the chromosome, speculate protein sequence, domain and function. DR_2566 gene was cloned successfully. Bio-informatic analysis results show that the target gene, with 507 bp full-length, encodes a protein with 168 amino acids and the theoretical molecular mass of this protein is about 19.136 KDa. Tertiary structure predicting indicates DR_2566 protein is a kind of endonuclease that can repair short DNA damage fragment. Full-length ORF of DR_2566 in D. Radiodurans was cloned successfully. Bio-informatic analysis indicated the DR_2566 protein might be involved in mismatch repairing function in D. Radiodurans.Cited

关键词

耐辐射球菌DR_2566基因基因克隆生物信息学

Keywords

Deinococcus radioduransDR_2566 geneGene cloningBioinformatics

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