Study on the down-regulation of PKCα by tamoxifen combined with <sup>60</sup>Co γ-irradiation in glioma cells

YIN Xiaoxiang; LIU Haiyan; FENG Shuang; MA Yan; ZHANG Haowen; LIU Fenju

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Study on the down-regulation of PKCα by tamoxifen combined with ⁶⁰Co γ-irradiation in glioma cells

更新时间：2023-03-27

- Study on the down-regulation of PKCα by tamoxifen combined with ⁶⁰Co γ-irradiation in glioma cells
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 1, Pages: 28-32(2011)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215123
  2.江苏省泰兴市人民医院放疗科　泰兴　225400
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YIN Xiaoxiang, LIU Haiyan, FENG Shuang, et al. Study on the down-regulation of PKCα by tamoxifen combined with ⁶⁰Co γ-irradiation in glioma cells. [J]. Journal of Radiation Research and Radiation Processing 29(1):28-32(2011)
DOI：

YIN Xiaoxiang, LIU Haiyan, FENG Shuang, et al. Study on the down-regulation of PKCα by tamoxifen combined with ⁶⁰Co γ-irradiation in glioma cells. [J]. Journal of Radiation Research and Radiation Processing 29(1):28-32(2011) DOI：

摘要

研究他莫昔芬（Tamoxifen，TAM）联合γ射线辐照对脑胶质瘤细胞PKCα蛋白表达的影响，初步探讨TAM降低辐射抗性的机制。采用Western-Blot法检测 PKCα和G,1,期调控蛋白CylinD,1,的表达；碱性单细胞凝胶电泳法检测DNA链断裂。结果提示，TAM联合γ射线辐照可诱导PKCα蛋白和CyclinD,1,蛋白表达量降低，增加单纯照射对DNA的损伤并抑制其修复。结果提示，TAM联合γ射线辐照能够增强TAM或辐照单独处理对PKCα蛋白表达的下调、诱导CyclinD1低表达和抑制DNA损伤修复，与TAM降低SHG-44细胞的辐射抗性有关。

Abstract

The mechanism of radio-sensitizing effect of tamoxifen on the influence of PKCα expression has been explored by tamoxifen combined with ,60,Co γ-irradiation in human glioma cells. The protein expression of PKCα and CyclinD1 were detected by using Western-Blot. The level of DNA strand breaks was detected by the alkaline single-cell gel electrophoresis assay. The results demonstrated that the combination of tamoxifen and ,60,Co γ-irradiation could reduce the expression of PKCα and CyclinD1.And it also enhanced the radiation-induced DNA damage and attenuated the repair of damaged DNA. The above results reveal that the combination of tamoxifen and ,60,Co γ-irradiation can enhance the downregulation of the expression of PKCα and the induction in the low expression of CyclinD1 as well as the delay of DNA repair. All these may be related to the impairment of the radioresistance of SHG-44 cells by tamoxifen.

关键词

他莫昔芬脑胶质瘤蛋白激酶CαCyclinD1；DNA损伤辐射抗性

Keywords

TamoxifenGliomaProtein Kinase Cα(PKCα)CyclinD1DNA damageRadioresistance

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Study on the down-regulation of PKCα by tamoxifen combined with 60Co γ-irradiation in glioma cells

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Study on the down-regulation of PKCα by tamoxifen combined with ⁶⁰Co γ-irradiation in glioma cells