Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer

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Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer

更新时间：2023-03-27

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- Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 1, Pages: 33-36(2011)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院放射医学与防护重点实验室　苏州　215123
  2.徐州市肿瘤医院　徐州　221002
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- 基金信息：
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ZHOU Yuanyuan, FENG Yang, ZHANG Xuguang, et al. Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer. [J]. Journal of Radiation Research and Radiation Processing 29(1):33-36(2011)
DOI：

ZHOU Yuanyuan, FENG Yang, ZHANG Xuguang, et al. Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer. [J]. Journal of Radiation Research and Radiation Processing 29(1):33-36(2011) DOI：

摘要

为研究青蒿琥酯联合照射对人宫颈癌HeLa和Siha细胞DNA损伤的影响，取对数生长期细胞，通过MTT比色法测定不同浓度青蒿琥酯作用24 h对HeLa和Siha细胞生长的抑制效应，将细胞分为单纯照射组和联合治疗组，每组分别给予0、2、4、6 Gy四个剂量的 ,60,Co γ射线照射，应用单细胞凝胶电泳(SCGE)法检测青蒿琥酯对HeLa和Siha细胞照射后DNA损伤的影响。MTT结果表明，青蒿琥酯对宫颈癌HeLa和Siha细胞的生长抑制呈剂量依赖性。单细胞凝胶电泳法检测发现：联合治疗组HeLa细胞经0、2、4和6 Gy ,60,Co γ射线辐照后彗星细胞的尾部DNA百分数及Olive尾矩明显大于单纯照射组，差别有统计学意义（,P,<,0.05），而Siha细胞联合治疗组与单纯辐照组相比，无明显差异。实验结果表明，青蒿琥酯对p53突变型子宫颈癌HeLa细胞具有明显的放射增敏作用，而对p53野生型子宫颈癌Siha细胞没有明显的放射增敏作用。

Abstract

In order to investigate the effect of artesunate combined with irradiaton on DNA damage of HeLa and Siha cells of human cervical cancer, HeLa and Siha cells were cultured in vitro and exposed to different concentration of artesunate for 24 h and MTT assay was used to observe the inhibitory effect of different concentrations of artesunate on the proliferation of HeLa and Siha cells. The cells were divided into 2 groups as the irradiated group and the union treatment group. Here it was set up four absorbed doses of ,60,Co γ-irradiation in each group with 0, 2, 4, and 6Gy, and the DNA damage were detected by single cell gel electrophoresis assay. MTT analysis showed that the inhibition of artesunate on HeLa and Siha cells of cervical cancer was in concentration-dependent manners. Single cell gel electrophoresis showed that the DNA damage of HeLa cells treated with artesunate was more serious than that treated only with irradiation (,P,<,0.05), but had no such effect on Siha cells. Artesunate can increase the radio-sensitivity of HeLa cells of cervival cancer with p53 mutant, but has no such effect on wide type p53 Siha cells.

关键词

青蒿琥酯放射增敏DNA损伤宫颈癌HeLa细胞

Keywords

ArtesunateRadiosensitivityDNA damageCervical cancer HeLa cell

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