The analysis of DNA repair genes expression in the blood of total body irradiated mice

LI Mingjuan; WANG Weiwei; CHEN Shiwei; CHENG Ying; MIN Rui

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The analysis of DNA repair genes expression in the blood of total body irradiated mice

更新时间：2023-03-27

- The analysis of DNA repair genes expression in the blood of total body irradiated mice
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 2, Pages: 93-98(2011)
- 作者机构：
  
  1.第二军医大学海军医学系放射医学教研室　上海　200433
  2.上海长海医院实验诊断科　上海　200433
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LI Mingjuan, WANG Weiwei, CHEN Shiwei, et al. The analysis of DNA repair genes expression in the blood of total body irradiated mice. [J]. Journal of Radiation Research and Radiation Processing 29(2):93-98(2011)
DOI：

LI Mingjuan, WANG Weiwei, CHEN Shiwei, et al. The analysis of DNA repair genes expression in the blood of total body irradiated mice. [J]. Journal of Radiation Research and Radiation Processing 29(2):93-98(2011) DOI：

摘要

观察小鼠全身 γ 射线辐照不同剂量、照射后不同时间，外周血细胞DNA损伤修复相关蛋白基因表达变化，筛选具有指示生物受照剂量潜力的指标。BALB/c小鼠，以0.8 和 1.6 Gy/min 两种剂量率，分别进行 0、2、4、6和8 Gy不同剂量照射。照射后4、8、12、24 和 48 h 眼眶取血，AxyPrep 试剂盒抽提全血细胞总mRNA，实时定量聚合酶链反应（Real-time quantitative polymerase chain reaction，RT-PCR）方法分析DNA损伤修复相关蛋白基因ATM（Ataxia telangiectasia mutated）、CDKN1A（Cyclin-dependent kinase inhibitor 1A）、DDB2（Damage-specific DNA-binding protein 2）和GADD45A（Growth arrest and DNA damage-inducible, alpha）转录水平。GraphPad Prism 5.0 software package（GraphPad Software，USA）进行数据统计分析。结果表明，与未照射对照组比，在2—8 Gy剂量范围，0.8 Gy/min剂量率照射条件下，照射后所有观察时间点，基因ATM、CDKN1A、DDB2和GADD45A表达均发生显著性变化，变化程度与受照剂量和照射后时间存在效应关系。相同照射剂量条件下剂量率增高，基因表达变化的幅度更明显。依据上述基因随照射后不同时间表达改变的信息，有可能在一定辐照剂量范围内，用DNA损伤修复蛋白基因表达指标估计生物体受照剂量大小。

Abstract

In this paper the expression changes of genes involved in DNA repair induced by radiation at different doses and post-irradaition time points has been investigated and the possibility of combining some genes as biomarkers for bio-dosimeter has been evaluated. The whole body of experimental mice was irradiated with 0, 2, 4, 6 and 8 Gy ,137,Cs γ-rays at 0.80 Gy/min and 1.60 Gy/min, respectively. Peripheral blood was collected at immediate, 4, 8, 12, 24 and 48 h after irradiation and RNA was extracted by AxyPrep Kit. The mRNA transcriptional expression levels of ATM, CDKN1A, DDB2 and GADD45A were detected by real-time quantitative polymerase chain reaction (RT-PCR). Expression changes of mRNA were compared with that of unirradiated control group at the dose rate of 0.8 Gy/min. A series of dose-and time-response of the changes were figured out. A heavy change in magnitude of genes transcription induced by irradiation at higher exposure dose rate could be found. The information from gene expression analysis as a greatly potential biomarkers may be used to indicate exposure doses at different post irradiation time points in a certain dose range.

关键词

电离辐射生物剂量计修复基因表达实时定量聚合酶链反应

Keywords

Ionizing radiationBiodosimeterGene expressionReal-time quantitative polymerase chain reaction (RT-PCR)

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