Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells

GAO Ling; LI Fengsheng; CHEN Xiaohua; LIU Qingjie

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Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells

更新时间：2023-03-27

- Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 2, Pages: 113-116(2011)
- 作者机构：
  
  1.中国疾病预防控制中心辐射防护与核医学安全所　北京　100088
  2.军事医学科学院放射与辐射医学研究所　北京　1000850
  3.第二炮兵总医院　北京　100088
- 作者简介：
- 基金信息：
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GAO Ling, LI Fengsheng, CHEN Xiaohua, et al. Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells. [J]. Journal of Radiation Research and Radiation Processing 29(2):113-116(2011)
DOI：

GAO Ling, LI Fengsheng, CHEN Xiaohua, et al. Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells. [J]. Journal of Radiation Research and Radiation Processing 29(2):113-116(2011) DOI：

摘要

为了探讨电离辐射对胃腺癌细胞中STAT3活性及定位的影响，首先利用Western Blot方法检测,60,Co γ 射线照射后胃癌BGC-823细胞内STAT3蛋白含量及活性变化，随后提取,60,Co γ 辐射前后BGC-823细胞核蛋白，检测细胞核内STAT3水平，最后利用免疫荧光染色进一步验证辐射前后STAT3在BGC-823细胞内的分布变化。结果发现，胃癌细胞BGC-823受到,60,Co γ 射线照射后，细胞内以及细胞核内磷酸化STAT3蛋白表达均降低，同时STAT3的入核减少。由此可见，辐射能够降低胃癌细胞内磷酸化STAT3的水平，从而使STAT3入核减少。此机制可能是放疗抑制胃癌细胞增殖、促进其凋亡的分子机制之一。

Abstract

This study is to investigate the effect of ionizing radiation on the activity and localization of STAT3 in gastric adenocarcinoma cells. After irradiated with ,60,Co γ-rays, the content and activity of STAT3 in gastric adenocarcinoma BGC-823 cells were detected by Western blot. The content of STAT3 in nucleus of BGC-823 was further detected by Western blot after the total protein in nucleus of irradiated BGC-823 was extracted. Finally, the distribution of p-STAT3,(Y705), in BGC-823 treated with or without irradiation was examined by immunofluorescence staining. The results showed that the level of p-STAT3,(Y705), in BGC-823 cells decreased significantly after ,60,Co γ-rays irradiation, and the content of STAT3 in BGC-823 cells nuclei also decreased after irradiation. As predicted, the p-STAT3 ,(Y705), localized in nucleus of BGC-823 cells also decreased significantly. ,60,Co γ-rays irradiation inhibited the phosphorylation of p-STAT3 ,(Y705), and further suppress the nucleus localization of STAT3, which might be involved in radiation-induced cell proliferation inhibition and apoptosis of gastric adenocarcinoma cells.

关键词

胃癌细胞电离辐射STAT3入核

Keywords

Gastric adenocarcinoma cellIonizing radiationSTAT3Nucleus localization

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