Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell

WANG Jichao; ZHANG Chao; CAI Jianming

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Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell

更新时间：2023-03-27

- Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 3, Pages: 159-162(2011)
- 作者机构：
  
  1.第二军医大学海军医学系放射医学教研室　上海　200433
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WANG Jichao, ZHANG Chao, CAI Jianming. Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell. [J]. Journal of Radiation Research and Radiation Processing 29(3):159-162(2011)
DOI：

WANG Jichao, ZHANG Chao, CAI Jianming. Effect of CpG-ODN combined with radiation on micronuclei cell of the human intestinal crypt epithelial cell. [J]. Journal of Radiation Research and Radiation Processing 29(3):159-162(2011) DOI：

摘要

研究了CpG-ODN（CpG oligonucleotides）对非免疫细胞人小肠隐窝上皮细胞（HIEC）照射后微核变化的影响。采用MTT法检测不同浓度的CpG-ODN对HIEC的细胞毒性，检测CpG-ODN预处理后的HIEC细胞在不同剂量辐射后微核率和微核细胞率的变化。结果表明，CpG-ODN在0.00–1.25 μmol/L浓度范围内对细胞的存活率没有显著影响，CpG-ODN能显著降低微核率和微核细胞率。实验结果提示CpG-ODN对HIEC有较小的毒性且能减少细胞辐照后的微核形成，具有一定的辐射保护作用。

Abstract

In order to study the changes of micronuclei cell frequency in the non-immune cell types, the human intestinal crypt epithelial cell (HIEC) was treated by CpG-ODN after radiation. MTT assay and micronuclei assay were used in this research. The result of MTT assay shows that CpG-ODN does not have any toxicity to HIEC in the concentration range of 0.00–1.25 μmol/L. Micronuclei assay measurement indicates that CpG-ODN can protect HIEC from radiation damage by reducing the micronucleus frequency (MNF) and the micronucleus cell frequency (MNCF). The experiment results reveal that CpG-ODN is safe and may have radioprotection effect on some non-immune human cell types.

关键词

CpG-ODN人小肠隐窝上皮细胞照射微核

Keywords

CpG-ODNHuman intestinal crypt epithelial cell(HIEC)IrradiationMicronucleus

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