Studies on the characteristics of DNA cleavage under the action of <sup>99m</sup>Tc(O)(PNA-dp)

SONG Hongtao; WEI Hongyuan; YANG Yuqing; WANG Guanquan; LUO Shunzhong

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Studies on the characteristics of DNA cleavage under the action of ^99mTc(O)(PNA-dp)

更新时间：2023-03-27

- Studies on the characteristics of DNA cleavage under the action of ^99mTc(O)(PNA-dp)
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 4, Pages: 214-218(2011)
- 作者机构：
  
  1.中国工程物理研究院核物理与化学研究所　绵阳　621900
- 作者简介：
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SONG Hongtao, WEI Hongyuan, YANG Yuqing, et al. Studies on the characteristics of DNA cleavage under the action of ^99mTc(O)(PNA-dp). [J]. Journal of Radiation Research and Radiation Processing 29(4):214-218(2011)
DOI：

SONG Hongtao, WEI Hongyuan, YANG Yuqing, et al. Studies on the characteristics of DNA cleavage under the action of ^99mTc(O)(PNA-dp). [J]. Journal of Radiation Research and Radiation Processing 29(4):214-218(2011) DOI：

摘要

肽核酸（PNA）分子具有特异基因靶向亲和性，用放射性核素标记后，可达到肿瘤靶向和对非靶组织最小损伤的目的，对于基因水平的肿瘤治疗具有十分重要的意义。本工作探索了,99m,Tc标记T基PNA单体所得配合物,99m,Tc（O）（PNA-dp）对常用基因靶向DNA的切割效果。结果表明，配合物在室温密封放置6 h，放化纯度大于90%，具有很好的稳定性；无溶血现象，可注射；在1 h即对质粒DNA表现出明显的切割效果，其机理应为,99m,Tc的射线辐射与PNA-dp的协同作用。

Abstract

Peptide nucleic acid can be used in the field of tumor gene therapy with minimal non-target damage if it binds to gene with high sequence specificity and selectivity when labeled with radionuclides. In this paper,99m,Tc (O) (PNA-dp) was synthesized, and the characteristics of DNA-cleavage were studied. It shows that the radiochemical purity remains over 90% after 6 h storage at room temperature, and the complex can be injected directly. Besides, the obvious DNA cleavage effect can be found after 1 h, and the mechanism of which maybe the cooperation of PNA-dp and the radiation of ,99m,Tc.

关键词

99mTc肽核酸DNA切割pUC 019 DNA

Keywords

99mTcPeptide nucleic acid (PNA)DNA cleavagepUC 019 DNA

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Studies on the characteristics of DNA cleavage under the action of 99mTc(O)(PNA-dp)

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Studies on the characteristics of DNA cleavage under the action of ^99mTc(O)(PNA-dp)