Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode

JIANG Qisheng; LI Zhiren; CHEN Feng; CAI Pengfei; LI Fengsheng; ZHU Haijing; ZHAGN Zhijie; TU Yu

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Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode

更新时间：2023-03-27

- Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode
- Journal of Radiation Research and Radiation Processing Vol. 29, Issue 5, Pages: 271-274(2011)
- 作者机构：
  
  1.第二炮兵总医院中心实验室　北京　100088
  2.苏州大学医学部放射医学与公共卫生学院　江苏省放射医学与防护重点实验室　苏州　215123
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JIANG Qisheng, LI Zhiren, CHEN Feng, et al. Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode. [J]. Journal of Radiation Research and Radiation Processing 29(5):271-274(2011)
DOI：

JIANG Qisheng, LI Zhiren, CHEN Feng, et al. Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode. [J]. Journal of Radiation Research and Radiation Processing 29(5):271-274(2011) DOI：

摘要

研究不同剂量氡暴露对大鼠基因甲基化和血清肿瘤标志物影响，探讨其在氡致肺癌早期诊断中的价值和意义。以HD-3型多功能生态氡室模拟不同浓度氡及其子体水平，30只染氡大鼠随机分为5组，即对照组、30工作水平月（WLM）组、60 WLM组、90 WLM组、120 WLM组，每组6只；用甲基化特异性PCR（MSP）方法，检测各组大鼠支气管肺泡灌洗液（BALF）细胞中p16和MGMT基因的甲基化率，ELISA方法检测各组染氡大鼠血清中常见肺癌标志物。结果发现，120 WLM组大鼠BALF细胞p16基因甲基化率为16.7%，血清CEA浓度明显高于其他各组（,P,<,0.05），NSE浓度显著高于对照组、30 WLM组及60 WLM组（,P,<,0.05）；各组大鼠BALF细胞MGMT基因甲基化率和血清CYFRA21-1浓度及p53抗体水平无显著差异（,P,>,0.05）。随着氡暴露剂量的增加，大鼠BALF细胞p16基因甲基化率及血清CEA、NSE浓度显著升高，为氡致肺癌早期肿瘤标志物的筛选提供了参考。

Abstract

In order to explore the primary diagnostic aspects of lung cancer the effects of radon on the genes methylation of p16 and MGMT in BALF cells and tumor markers in serum of rats were investigated. 30 male wistar rats were randomly and equally divided into 5 groups: control group, 30 working level month (WLM) group, 60 WLM group, 90 WLM group and 120 WLM group, respectively. The rats were exposed to multi-functional ecology radon cabinet except for control group. Methylation-specific PCR (MSP) was used to detect the methylation rates of p16 gene and MGMT gene in bronchoalveolar lavage fluid (BALF) cells, ELISA was used to test the concentration of lung cancer biomarkers in serum of rats. The results showed that the methylation rates of p16 gene and the level of CEA in 120 WLM group increased significantly compared to other groups (,P,<,0.05). The level of NSE in 120 WLM group was remarkably higher than that in other groups except for 90 WLM group (,P,<,0.05), but the methylation rate of MGMT gene and the concentration of CYFRA21-1 and p53 antibody did not change significantly among all the 5 groups (,P,>,0.05). The above results suggested that it might be benefit to screen biomarker of lung cancer induced by high concentration radon exposure as the methylation rates of p16 gene and the levels of CEA and NSE increased significantly in high dose radon exposed rats.

关键词

氡基因甲基化肿瘤标志物

Keywords

RadonGene methylationTumor markers

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