THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION(LDR) ON NK ACTIVITY

LÜ Duicai; SU Liaoyuan

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THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION(LDR) ON NK ACTIVITY

更新时间：2023-03-30

- THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION(LDR) ON NK ACTIVITY
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 3, Pages: 218-221(2003)
- 作者机构：
  
  1.航天医学工程研究所　北京　100094
  2.苏州大学放射医学与公共卫生学院医学放射生物学教研究室　苏州　215007
- 作者简介：
- 基金信息：
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LÜ Duicai, SU Liaoyuan. THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION(LDR) ON NK ACTIVITY. [J]. Journal of Radiation Research and Radiation Processing 21(3):218-221(2003)
DOI：

LÜ Duicai, SU Liaoyuan. THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION(LDR) ON NK ACTIVITY. [J]. Journal of Radiation Research and Radiation Processing 21(3):218-221(2003) DOI：

摘要

研究了低剂量辐射（LDR）后脾细胞裂解成分对淋巴细胞自然杀伤细胞（NK）活性的影响。用McAb直接法分离出CD,57,细胞和非CD,57,细胞(除CD,57,外的所有淋巴细胞)并以两者为效应细胞，K5,62,细胞为靶细胞，将CD,57,与非CD,57,杀伤肿瘤细胞的作用进行比较；测定计算各自的杀伤活性并观察LDR辐射后脾细胞裂解成分对杀伤活性的影响。结果表明， CD,57,及非CD,57,细胞均对肿瘤细胞有杀伤作用，且非CD,57,细胞的作用更强；LDR辐射后脾细胞裂解成分能分别增强CD,57,及非CD,57,细胞体外抗肿瘤细胞效应(NK细胞活性及NK活性)。

Abstract

To find effect of lysate of spleen cells after LDR on NK activity of CD,57, cells or non-CD,57, cells. Lysate of spleen cells after LDR were extracted. McAb (anti CD,57, cells) was used to separate CD,57, cells from human peripheral blood by Panning direct method. The CD,57, cells and non-CD,57, cells were used as effective cells.K,562, cells labeled by ,3,H-TdR were used as target cells. The ratio of effect cells to target cells was 10:1.NK activity of CD,57, cells or non-CD,57, cells with the lysate of spleen cells after LDR was reflected by the efficiency of anti tumor cells. The 3H-TdR incorporation in K,562, cells cultured with non-CD,57, cells was significantly lower than that with CD,57, cells. After use of the lysate of spleen cells after LDR, NK activities of NK cells and non-NK cells were 1.24 and 1.58 respectively. They were both increased obviously compared with control groups. The effect of anti K,562, cells of non-NK cells is even greater than that of NK cells. The lysate of spleen cells after LDR can enhance the effect of both non-NK cells and NK cells.

关键词

低剂量辐射脾细胞裂解成分淋巴细胞NK 活性

Keywords

Low dose radiation (LDR)Lysate of spleen cellsLymphocyteNK activity

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