THE HYPERTHERMIA RADIOSENSITIZATION OF HUMAN GLIOMA CELLS SHG-44 AND THE EXPRESSION OF FAS/FASL

ZHOU Juying; TU Yu; YU Zhiying; SU Liaoyuan; XU Changshao

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THE HYPERTHERMIA RADIOSENSITIZATION OF HUMAN GLIOMA CELLS SHG-44 AND THE EXPRESSION OF FAS/FASL

更新时间：2023-03-30

- THE HYPERTHERMIA RADIOSENSITIZATION OF HUMAN GLIOMA CELLS SHG-44 AND THE EXPRESSION OF FAS/FASL
- Journal of Radiation Research and Radiation Processing Vol. 21, Issue 3, Pages: 222-224(2003)
- 作者机构：
  
  1.苏州大学附属第一院肿瘤放射治疗科　苏州　215006
  2.苏州大学放射医学与公共卫生学院　苏州　215006
- 作者简介：
- 基金信息：
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ZHOU Juying, TU Yu, YU Zhiying, et al. THE HYPERTHERMIA RADIOSENSITIZATION OF HUMAN GLIOMA CELLS SHG-44 AND THE EXPRESSION OF FAS/FASL. [J]. Journal of Radiation Research and Radiation Processing 21(3):222-224(2003)
DOI：

ZHOU Juying, TU Yu, YU Zhiying, et al. THE HYPERTHERMIA RADIOSENSITIZATION OF HUMAN GLIOMA CELLS SHG-44 AND THE EXPRESSION OF FAS/FASL. [J]. Journal of Radiation Research and Radiation Processing 21(3):222-224(2003) DOI：

摘要

观测加热联合放射对 SHG−44细胞的影响及相关基因Fas/FasL的表达。采用细胞集落法及流式细胞术观察加热对脑胶质瘤细胞SHG−44放射后集落形成的影响和凋亡的变化，并用RT−PCR法检测Fas/FasL表达。结果表明，放射+42℃加热（加热时间40min）较单纯放射（2Gy）的细胞集落明显减少，热增强比(TER)为1.95；空白对照组、单纯放射组、放射+加热组凋亡比率分别为：20.2、29.3、59.1；Fas在3个实验组中均表达，但FasL仅在单纯放射组、放射+加热组中表达。热能明显增加脑胶质瘤细胞SHG−44的放射敏感性，也表明凋亡与Fas/FasL有关。

Abstract

The effects of hyperthermia and radiation on human glioma SHG−44 cells and the alteration of Fas/FasL expression in the treated cells were investigated. The sensitivity of SHG−44 cells to hyperthermia and radiation was measured by colony forming assay and flow cytometry (FCM). The expression of Fas/FasL was detected by RT−PCR. By hyperthermia ( 42°C, 40min) combined irradiation, the colony forming ratio was reduced in a more effective way than by just the irradiation. The TER was 1.95. The apoptosis ratios were 20.2, 29.3, 59.1%, respectively, for the control group , the just-irradiation group and the hyperthermia combined radiation group. The expression of Fas was found in the three groups, but the expression of FasL could be found only in the two treatment groups. Hyperthermia could boost the effectiveness of radiation against glioma cells significantly. This also indicates some relationships between apoptosis and Fas/FasL.

关键词

脑胶质瘤细胞放射加热Fas/FasL

Keywords

Human Glioma CellsRadiationHyperthermiaFas/FasL

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The Second Affiliated Hospital of Soochow University

Beijing Institute of Radiation Medicine

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Norman Bethune University of Medical Sciences

1（复旦大学附属肿瘤医院肿瘤研究所复旦大学上海医学院肿瘤学系）

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