Changes in mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation

Wang Xiaomei; Ju Guizhi; Fu Haiqing; Mei shujiang; Liu Shuzheng

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Changes in mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation

更新时间：2023-03-30

- Changes in mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 1, Pages: 43-46(2004)
- 作者机构：
  
  1.汕头大学医学院生物教研室　汕头　515031
  2.吉林大学公共卫生学院放射生物教研室　长春　130021
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Wang Xiaomei, Ju Guizhi, Fu Haiqing, et al. Changes in mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation. [J]. Journal of Radiation Research and Radiation Processing 22(1):43-46(2004)
DOI：

Wang Xiaomei, Ju Guizhi, Fu Haiqing, et al. Changes in mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation. [J]. Journal of Radiation Research and Radiation Processing 22(1):43-46(2004) DOI：

摘要

采用流式细胞术和半定量RT-PCR的方法分别检测4Gy X射线照射后EL-4细胞不同细胞周期时相和EL-4细胞内cyclin B1及cdc2 mRNA水平的时程变化，以探讨X射线照射诱导EL-4细胞G,2,期阻滞的分子调控机制。结果表明，4Gy X射线照射EL-4细胞后2h，G,2,期细胞开始明显增多，4—8h达峰值（,p,<,0.001）。EL-4细胞内cyclin B1 mRNA水平于照射后1h开始下降，24h降至最低值，为假照组的57.7 %，72h恢复至正常水平。EL-4细胞中cdc2 mRNA水平于照射后12h开始降低，24—48h降至最低水平，分别为假照组的59.2%和58.9%， 72h恢复至假照组水平。结果提示, 4Gy X射线照射可诱导EL-4细胞cyclin B1及cdc2 mRNA水平明显下降，直接导致MPF活性减少是引发G,2,期阻滞的关键。

Abstract

To explore the molecular mechanism of G,2, arrest of EL-4 cells induced by X-irradiation, flow cytometry (FCM) and semi-quantity RT-PCR methods were used to observe the time-course changes of cell cycle and the mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation. Results showed that the positive cells in G,2,/M increased at 2h and reached the maximum at 4—8h after the irradiation (,p,<,0.001). The cyclin B1 mRNA level of El-4 cells began decrease at 1h, reached the minimum at 24h after the irradiation. Meanwhile, the cdc2 mRNA level also declined during 12h to 48h, reduced to 59.2 %and 58.9% compared with that of control group respectively, and recovered at 72h after the irradiation. The results suggests that MPF(M phase promoting factor) activity reduction resulted from inhibition of cdc2 and cyclin B play a critical role in G,2, arrest induced by X-ray irradiation in EL-4 cells.

关键词

电离辐射EL-4细胞cyclin Bcdc2

Keywords

Ionizing radiationEL-4 cellscyclin Bcdc2

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