MEK inhibitor u0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells

GU Qingyang; WANG Dewen; WANG Lisheng; DENG Hua; LIU Jie; PENG Ruiyun; DONG Bo; GAO Yabing; WANG Xiaomin

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MEK inhibitor u0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells

更新时间：2023-03-30

- MEK inhibitor u0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 1, Pages: 39-42(2004)
- 作者机构：
  
  1.军事医学科学院放射医学研究所　北京　100850
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GU Qingyang, WANG Dewen, WANG Lisheng, et al. MEK inhibitor u0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells. [J]. Journal of Radiation Research and Radiation Processing 22(1):39-42(2004)
DOI：

GU Qingyang, WANG Dewen, WANG Lisheng, et al. MEK inhibitor u0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells. [J]. Journal of Radiation Research and Radiation Processing 22(1):39-42(2004) DOI：

摘要

为研究bFGF（碱性成纤维细胞生长因子）对辐射诱导的血管内皮细胞凋亡的作用及其信号转导途径，以,60,Coγ射线照射法建立人脐静脉内皮细胞株ECV304细胞凋亡模型，以流式细胞术（Annexin-V-FITC+PI标记）检测细胞凋亡率。观察不同浓度的bFGF和MEK的特异性抑制剂U0126对细胞凋亡的影响。结果显示，bFGF对辐射诱导的ECV304细胞凋亡有明显的抑制作用，而U0126可阻断bFGF的抗凋亡作用。提示bFGF可能通过MAPK信号转导途径抑制辐射诱导的血管内皮细胞凋亡。

Abstract

In order to investigate the effect of bFGF on radiation-induced apoptosis in vascular endothelial cells and its signal transduction pathways, ECV304 cells were irradiated with ,60,Co γ rays for establishing cell apoptosis model. Flow cytometry with Annexin-V-FITC-PI labeling was used to determine cell apoptosis. Different amounts of bFGF and MEK inhibitor U0126 were used to study their effects on radiation-induced endothelial cell apoptosis. It was found that bFGF could inhibit radiation-induced apoptosis in ECV304 cells, but U0126 could block the anti-apoptosis effect of bFGF, suggesting that bFGF may inhibit radiation-induced apoptosis in vascular endothelial cells through of MAPK signaling.

关键词

bFGF血管内皮细胞凋亡MEKU0126

Keywords

bFGFvascular endothelial cellsapoptosisMEKU0126

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