Senescence associated β-galactosidase expressed by tumor cells after low-dose-rate β-irradiation

DING Weimin; TIAN Jiahe; ZHANG Jinming; FENG Huiru; HE Yijie; SHAO Mingzhe

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Senescence associated β-galactosidase expressed by tumor cells after low-dose-rate β-irradiation

更新时间：2023-03-30

- Senescence associated β-galactosidase expressed by tumor cells after low-dose-rate β-irradiation
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 1, Pages: 51-55(2004)
- 作者机构：
  
  1.中国人民解放军总医院核医学科　北京　100853
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DING Weimin, TIAN Jiahe, ZHANG Jinming, et al. Senescence associated β-galactosidase expressed by tumor cells after low-dose-rate β-irradiation. [J]. Journal of Radiation Research and Radiation Processing 22(1):51-55(2004)
DOI：

DING Weimin, TIAN Jiahe, ZHANG Jinming, et al. Senescence associated β-galactosidase expressed by tumor cells after low-dose-rate β-irradiation. [J]. Journal of Radiation Research and Radiation Processing 22(1):51-55(2004) DOI：

摘要

用,32,P放射性贴片照射HeLa细胞，细胞化学染色和生长曲线校正相结合检测照射后表达衰老相关性β-半乳糖苷酶（SA-β-Gal）的细胞及其数量变化趋势。结果表明，照射后，部分肿瘤细胞可以逆转进入表达SA-β-Gal的死亡相M1 或M2。由照射诱发的染色阳性细胞的绝对数量在观察时间内持续增长，并且，在一定剂量率范围内（7.5—60cGy/h）的等剂量照射后，低剂量率β射线可以诱发更多的SA-β-Gal阳性细胞。此外，诱发衰老是核素内照射治疗肿瘤中存在的远期治疗效应的可能机制。

Abstract

HeLa cells were irradiated with ,32,P radioactive filter papers. Cells expressing senescence associated β-galactosidase(SA-β-Gal) were quantified. And to acquire information about the absolute numbers of SA-β-Gal+ cells and their changing tendency, the rates of cells expressing SA-β-Gal were corrected with cell growth curves. Results show that after low-dose-rate β irradiation, some tumor cells can reverse into mortality stage M,1, or M,2, cells which express SA-β-Gal. Numbers of SA-β-Gal+ cells induced by the irradiation were increased. And in dose rates of 7.5—60cGy/h, more SA-β-Gal+ cells could be induced by the irradiation than higher dose-rate irradiations at the same dose. Moreover, it may indicate that long-term therapeutic effect of internal irradiations could be resulted by induced aging of tumor cells .

关键词

β射线低剂量率细胞衰老β-半乳糖苷酶HeLa细胞

Keywords

β-rayslow-dose-ratecell agingbeta-galactosidaseHeLa cells

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