Effect of low doses of γ−rays to challenge doses of MMC on the splenic lymphocyte in mice

MENG Qingyong; GAO Lili; XU Meiyi

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Effect of low doses of γ−rays to challenge doses of MMC on the splenic lymphocyte in mice

更新时间：2023-03-30

- Effect of low doses of γ−rays to challenge doses of MMC on the splenic lymphocyte in mice
- Journal of Radiation Research and Radiation Processing Vol. 22, Issue 4, Pages: 253-256(2004)
- 作者机构：
  
  1.广东医学院分析中心　湛江　524023
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MENG Qingyong, GAO Lili, XU Meiyi. Effect of low doses of γ−rays to challenge doses of MMC on the splenic lymphocyte in mice. [J]. Journal of Radiation Research and Radiation Processing 22(4):253-256(2004)
DOI：

MENG Qingyong, GAO Lili, XU Meiyi. Effect of low doses of γ−rays to challenge doses of MMC on the splenic lymphocyte in mice. [J]. Journal of Radiation Research and Radiation Processing 22(4):253-256(2004) DOI：

摘要

探讨低剂量辐射诱导哺乳动物抗丝裂霉素C （Mitomycin C，MMC）损伤的免疫适应性反应。小鼠接受低剂量γ射线作用后6h，再给予损伤剂量的MMC，然后通过脾细胞计数、流式细胞术、Con A和LPS刺激的小鼠脾淋巴细胞转化观察小鼠脾细胞数、细胞周期和DNA含量的变化。1mg/kg的MMC（B）组小鼠脾细胞数明显低于正常对照（A）组，50—100mGy照射加MMC（C、D、E）组小鼠脾细胞数明显高于B组。Con A刺激的小鼠脾淋巴细胞转化细胞周期分析表明，D组、E组G,0,/G,1,期和S期细胞相对百分率分别显著低于和高于B组。而LPS刺激组，除E组G,0,/G,1,期外，D组、E组G,0,/G,1,期和S期细胞相对百分率也分别明显低于和高于B组。低剂量γ射线可以诱导MMC损伤小鼠脾细胞数和细胞周期进程的适应性反应。

Abstract

To investigate the adaptive response of low doses of gamma−rays to challenge doses of Mitomycin C(MMC) on the splenic lymphocytes in mammalian, mice were irradiated with inductive doses of 50, 75, 100mGy 6h before challenge dose of MMC (1mg/kg). Observations were made on efficacy of splenocyte count, cell cycle progression, DNA synthesis in number of splenocytes, Flow cytometry, the splenic lymphocyte transformation induced by Con A and LPS. The number of splenocytes in Group B(1mg/kg of MMC) was significantly lower than that the control(Group A). The number of splenocytes in Group C, D and E (50−100mGy + MMC) was significantly higher than that in Group B. Both the percentages of G,0,/G,1, and S phase cells in Group D and E were lower and higher than that in Group B in cell cycle progression of the splenic lymphocyte transformation induced by Con A. Significant changes of the numbers were found in the LPS treated groups (except G,0,/G,1, phase of Group E). The results showed adaptive response of low doses of γ−rays to challenge doses of MMC on number of splenocytes and cell cycle progression of the splenic lymphocyte transformation induced by Con A and LPS in mice.

关键词

低剂量γ射线抗丝裂霉素C适应性反应细胞周期脾淋巴细胞转化小鼠

Keywords

Low dose γ−raysMitomycin CAdaptive responseCell cycle progressionSplenic lymphocyte transformationMice

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