Radiation effects of electromagnetic pulses on mouse blood-testis barrier

HOU Wugang; ZHAO Jie; ZHANG Yuanqiang; ZENG Lihua; GUO Guozhen

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Radiation effects of electromagnetic pulses on mouse blood-testis barrier

更新时间：2023-03-30

- Radiation effects of electromagnetic pulses on mouse blood-testis barrier
- Journal of Radiation Research and Radiation Processing Vol. 23, Issue 6, Pages: 366-370(2005)
- 作者机构：
  
  1.第四军医大学基础部组织学与胚胎学教研室　西安　710032
  2.第四军医大学预防医学系放射医学教研室　西安　710032
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HOU Wugang, ZHAO Jie, ZHANG Yuanqiang, et al. Radiation effects of electromagnetic pulses on mouse blood-testis barrier. [J]. Journal of Radiation Research and Radiation Processing 23(6):366-370(2005)
DOI：

HOU Wugang, ZHAO Jie, ZHANG Yuanqiang, et al. Radiation effects of electromagnetic pulses on mouse blood-testis barrier. [J]. Journal of Radiation Research and Radiation Processing 23(6):366-370(2005) DOI：

摘要

采用常规苏木精−伊红染色（Hematoxylin−Eosin staining，HE staining）、硝酸镧示踪电镜和埃文思兰（Evans Blue，EB）尾静脉注射三种方法，综合观察100kV/m和400kV/m两种不同强度电磁脉冲辐射对小鼠睾丸血−睾屏障的影响。三种方法观察结果基本一致，两种强度的电磁脉冲辐射均可造成小鼠睾丸支持细胞和血−睾屏障不同程度的损伤，400kV/m辐射组损伤更甚，辐射后1d即可见大量生精细胞的凋亡与坏死，支持细胞亦发生形态结构的变化，血−睾屏障严重损伤，随后大量第Ⅷ期生精小管内出现基底室与近腔室的分离，大量凋亡与坏死的生精细胞向管腔的排放，至辐射后21d仍可见到血−睾屏障损伤；100kV/m辐射组的损伤较400kV/m辐射组稍轻，辐射后14d支持细胞、生精上皮以及血−睾屏障的损伤已基本恢复。结果提示一定强度的电磁脉冲可以造成小鼠血−睾屏障的损伤，这种损伤呈辐射强度依赖性，并且与损伤后恢复时间相适应。

Abstract

Radiation effects caused by 100kV/m and 400kV/m electromagnetic pulse (EMP) irradiations on mouse blood−testis barrier were studied by means of routine HE staining, Lanthanum traced electron microscope and injection of caudal vein with Evans Blue. The EMP irradiation of different dose rates damaged Sertoli's cell and blood−testis barrier of mouse testis in different levels. Severe injuries were observed with the 400kV/m irradiation group, with apoptosis and necrosis in a large quantity of the spermatogenic cells, shape and structural changes of the Sertoli's cells, and serious injuries to the blood−testis barrier, one day after the irradiation. The basal compartment separated from the adluminal compartment in most of the = 8 \* ROMAN VIII stage seminiferous epithelium, and a great number of apoptosis and necrosis spermatogenic cells were released into the cavities. Injuries of blood−testis barrier could be observed 21days after the 400kV/m irradiation. The injuries of 100kV/m irradiation groups were less severe than the 400kV/m groups, in which the damages to the Sertoli's cells, the seminiferous epithelium and blood−testis barrier recovered to some extent 14days after the irradiation. We conclude that EMP irradiation can damage mouse blood−testis barrier. The injuries, and the time for recovery, are related to EMP power intensity.

关键词

电磁脉冲辐射血−睾屏障支持细胞埃文思兰硝酸镧睾丸

Keywords

Electromagnetic pulse irradiationBlood−testis barrierSertoli's cellEvans BlueLanthanumTestis

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