Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study

ZHANG Junxiang; KONG Zhaolu; SHEN Zhifen; TONG Shungao; JIN Yizun

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Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study

更新时间：2023-03-30

- Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 2, Pages: 107-110(2006)
- 作者机构：
  
  1.复旦大学放射医学研究所　上海　200032
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ZHANG Junxiang, KONG Zhaolu, SHEN Zhifen, et al. Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study. [J]. Journal of Radiation Research and Radiation Processing 24(2):107-110(2006)
DOI：

ZHANG Junxiang, KONG Zhaolu, SHEN Zhifen, et al. Radiosensitivity of a monoclonal human lung adenocarcinoma cell line with MDR phenotype induced by CDDP: an in vitro study. [J]. Journal of Radiation Research and Radiation Processing 24(2):107-110(2006) DOI：

摘要

对顺铂（Cisplatin，CDDP）诱导的人肺腺癌多药耐药单克隆细胞株SPC−A−1/CDDP−4 及其亲代SPC−A−1细胞体外的辐射敏感性进行研究。分别在有氧和乏氧条件下检测 SPC−A−1/CDDP−4 细胞和SPC−A−1细胞内谷胱甘肽（Glutathione，GSH）的含量，对SPC−A−1/CDDP−4细胞和SPC−A−1细胞体外的辐射敏感性进行研究，观察GSH生物合成抑制剂丁胱亚磺酰亚胺（Buthionine sulfoximine，BSO）对SPC−A−1/CDDP−4细胞和SPC−A−1细胞的辐射增敏作用。实验结果表明：单克隆细胞株 SPC−A−1/CDDP−4不仅对化疗药： CDDP、阿霉素（Adriamycin，ADM）、甲氨蝶呤（Methotrexate，MTX）和长春新硷（Vincristine，VCR）具有多药耐药性，而且对,137,Cs γ 射线也具有一定的交叉耐受性；这可能与SPC−A−1/CDDP−4 细胞的 GSH 含量无论是在有氧或乏氧条件下，均高于亲代的SPC−A−1细胞有关；BSO在有氧和乏氧条件下对 SPC−A−1/CDDP−4细胞和SPC−A−1细胞均有辐射增敏作用，但是乏氧条件下的辐射增敏作用大于有氧，对SPC−A−1/CDDP−4细胞的辐射增敏作用大于对SPC−A−1细胞的辐射增敏作用。

Abstract

The study was to evaluate radiosensitivity of a monoclonal human lung adenocarcinoma cell line SPC−A−1/CDDP−4 with MDR phenotype induced by cisplatin (CDDP) compared with its parental cell SPC−A−1 in vitro. The glutathione (GSH) content and the radiosensitivity of SPC−A−1/CDDP−4 and SPC−A−1 cells were investigated in aerobic and under hypoxia, respectively. The radiosensitization effect of buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, to SPC−A−1/CDDP−4 and SPC−A−1 cells was observed. The results indicated that the monoclonal human lung adenocarcinoma cell line SPC−A−1/CDDP−4 showed, to some extent, a cross−resistance to ,137,Cs γ−ray, in addition to its resistance to anticancer drugs (CDDP, ADM, MTX and VCR). The GSH content of SPC−A−1/CDDP−4 cells was higher than that of SPC−A−1 cells both in aerobic and under hypoxia which might account for it. BSO had radiosensitization effect to SPC−A−1/CDDP−4 and SPC−A−1 cells both in aerobic and under hypoxia, but it was stronger under hypoxia than in aerobic and it was stronger to SPC−A −1/ CDDP−4 cells than to SPC−A−1 cells.

关键词

辐射敏感性多药耐药性谷胱甘肽丁胱亚磺酰亚胺乏氧

Keywords

RadiosensitivityMultidrug resistance(MDR)Glutathione (GSH)Buthionine sulfoximine (BSO)Hypoxia

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