Screening on the higher yield of transaminase producing <italic style="font-style: italic">Escherichia coli</italic> with the metal ion implantation

ZHANG Ning; YU Long; YANG Lifeng

您当前的位置：

首页 >

文章列表页 >

Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation

更新时间：2023-03-30

- Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 2, Pages: 125-128(2006)
- 作者机构：
  
  1.南京工业大学制药与生命科学学院　南京　210009
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
ZHANG Ning, YU Long, YANG Lifeng. Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation. [J]. Journal of Radiation Research and Radiation Processing 24(2):125-128(2006)
DOI：

ZHANG Ning, YU Long, YANG Lifeng. Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation. [J]. Journal of Radiation Research and Radiation Processing 24(2):125-128(2006) DOI：

摘要

利用金属钛离子注入天冬氨酸转氨酶生产菌株U12-3-19进行诱变，摸索金属离子与该生产菌株的互相作用规律，统计变异情况，并与氮离子注入诱变和紫外诱变的效果做了比较，得到了较高的突变率和较广的突变谱。通过大量筛选，在苯丙酮酸的浓度为20g/L的条件下，得到了使L-苯丙氨酸产量提高22.67%的高产菌株U12-25-116-J75。

Abstract

Mutagenic effects of the transaminase producing bacterium ,Escherichia coli, U12-3-19 by low energy metal ion (Ti,2+, ) implantation were investigated. And the different mutagenic effects of ,E.coli, irradiated with UV and low-energy ions were compared. The results showed that the ,E.coli, after the ion implantation could achieve higher rate of mutation and wide mutational spectrum. A high yield L-phe strain has been bred from this. In the shaking flask, the L-phe productivity of strain U12-25-116-J75 increased by 22.67% than of the comparison strain with the PPA concentration of 20g/L.

关键词

金属离子注入诱变选育L-苯丙氨酸

Keywords

Metal ion implantationMutationBreedL-phenylalanine

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Breeding of citric acid-producing bacteria by 15 keV N⁺ ion implantation

Mutagenesis of Clostridium acetobutylicum by HEPE and research of the fermentation

Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1

Mutation of Blakeslea trispora in lycopene production by ion beams

Screening on the high yield validamycin producing strain by implantationwith N⁺ and Ti⁺ ion sources

Related Author

No data

Related Institution

College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology

Jiangsu TV University

School of Life Sciences and Technology, Tongji University

Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Institute of Modern Physics, Chinese Academy of Sciences

⁰

Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation

DOI：

摘要

Abstract

关键词

Keywords

references