Study on ultrastructural changes in thyroid gland of rats exposed to pulsed electromagnetic wave

ZHOU Xiaoguang; ZENG Guiying; REN Dongqing; FANG Henghu; SU Xiaoming; HUANG Xiaofeng

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Study on ultrastructural changes in thyroid gland of rats exposed to pulsed electromagnetic wave

更新时间：2023-03-30

- Study on ultrastructural changes in thyroid gland of rats exposed to pulsed electromagnetic wave
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 2, Pages: 120-124(2006)
- 作者机构：
  
  1.第四军医大学军事预防医学系放射医学教研室　西安710032
  2.第四军医大学基础部电子显微镜中心　西安710032
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ZHOU Xiaoguang, ZENG Guiying, REN Dongqing, et al. Study on ultrastructural changes in thyroid gland of rats exposed to pulsed electromagnetic wave. [J]. Journal of Radiation Research and Radiation Processing 24(2):120-124(2006)
DOI：

ZHOU Xiaoguang, ZENG Guiying, REN Dongqing, et al. Study on ultrastructural changes in thyroid gland of rats exposed to pulsed electromagnetic wave. [J]. Journal of Radiation Research and Radiation Processing 24(2):120-124(2006) DOI：

摘要

研究脉冲电磁波（Pulsed electromagnetic wave，PEMW）对大鼠甲状腺形态及功能的影响。雄性SD (Sparague-dawley rats，多雷大鼠) 大鼠固定于场强为115kV m,-1,的脉冲电磁场中进行1.2万次脉冲的全身辐照，分别于辐照后24、48、96、192h采血、分离血清、应用放射免疫分析方法（Radioimmunoassay，RIA）测定大鼠血清中促甲状腺激素（Thyroid-stimulating hormone，TSH）、甲状腺素（Thyroxine，,T,4,）及三碘甲腺原氨酸（Triiodo-thyronine，,T,3,）的含量；同时取甲状腺，制作常规光镜、电镜标本，分别在光学显微镜和透射电子显微镜下观察甲状腺形态的变化。脉冲电磁波辐照后24h，大鼠血清,T,3,、,T,4,、TSH均迅速上升至最高点，其中,T,4,、TSH与假辐照组比较均有显著性差异（,p,<,0.01）；随后激素含量逐渐降低，并呈波动性变化。光镜下，各辐照组大鼠甲状腺组织结构未见明显改变；在电子显微镜下，脉冲电磁波辐照后12h大鼠甲状腺滤泡上皮细胞即出现超微结构改变，至48h呈逐渐加重趋势，主要表现为内质网明显扩张、大量蛋白分泌物聚集，脂滴出现，核异染色质的边集等；至照后96h损伤减轻，但尚未完全恢复正常。脉冲电磁波辐照可引起大鼠血清中TSH、,T,3,、,T,4,含量变化和甲状腺超微结构的损伤性改变，其超微结构的损伤主要累及粗面内质网。

Abstract

The work is to observe effects of PEMW (pulse electromagnetic wave) exposure on function and morphology of thyroid gland of rats. At different time points (24, 48, 96 and 192h) after exposure to PEMW (E=115kV m,-1, 12000 pulses), radioimmunoassay was performed to observe the levels of thyroid-stimulating hormone (TSH), thyroxine (,T,4,) and triiodo- thyronine (,T,3,) in sera of the male Sparague-Dawley rats. Optic microscopy and transmission electron microscopy (TEM) were used to observe structural changes of the thyroid gland at the time points. The ,T,3,、,T,4, and TSH in sera increased significantly, reaching a peak at 24h and decreasing gradually then. Although no obvious changes in the thyroid gland were observed under the optic microscope, some ultrastructral changes in the thyroid gland were found under the TEM. The ultrastructures were obviously changed at 12h and aggravated until 48h. In the experimented rats, dilatated endoplasmic reticulum gathered with lots of protein excretion, lipid droplet and heterochromatin gathered under the nucleus membranes were observed in follicular epithelial cells. These changes were palliated at 96h but not recovered. PEMW can affect levels of hormones in sera and the hazard ultrastructual changes of thyroid gland. Endoplasmic reticulum is the main injured organelle.

关键词

脉冲电磁波甲状腺大鼠激素形态

Keywords

Pulse electromagnetic wave(PEMW)Thyroid glandRatHormoneMorphous

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