Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray

CHEN Ying; LUO Yisheng; CAO Zhenshan; LIU Xiulin

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Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray

更新时间：2023-03-30

- Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 4, Pages: 241-244(2006)
- 作者机构：
  
  1.军事医学科学院放射与辐射医学研究所　北京　100850
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CHEN Ying, LUO Yisheng, CAO Zhenshan, et al. Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray. [J]. Journal of Radiation Research and Radiation Processing 24(4):241-244(2006)
DOI：

CHEN Ying, LUO Yisheng, CAO Zhenshan, et al. Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray. [J]. Journal of Radiation Research and Radiation Processing 24(4):241-244(2006) DOI：

摘要

为准确估算大剂量事故受照者的生物剂量，拟合6—22Gy照射后染色体畸变剂量效应曲线。采用,60,Co γ射线照射离体人血，浓集有核细胞，一步法培养52h、68h和72h收获制片。计数双（多）着丝点+环数目，比较不同培养时间畸变差异，拟合剂量效应曲线及数学方程。用大剂量事故受照者的剂量结果对曲线进行验证。实验表明，大剂量照射后需适当延长细胞培养时间，52—72h获得的染色体双+环畸变率无明显差异。新拟合的剂量效应曲线符合线性平方模型,Y,=-2.269+0.776D-7.868×10,-3,D,2,。经事故剂量验证结果可靠。本研究首次建立了6—22Gy照射后染色体双+环剂量效应曲线及数学方程，为特大剂量照射后的准确剂量估算提供了可依据的方法。

Abstract

To estimate accurately biological dose of the victims exposed to high dose, the dose-response curves of chromosome aberration induced by 6—22Gy ,60,Co γ-ray were established. Human peripheral blood in vitro was irradiated, then lymphocytes were concentrated, cultured 52h, 68h and 72h and harvested. The frequencies of dicentrics (multi-centrics) and rings were counted and compared between different culture times The dose-response curves and equations were established, as well as verified with high dose exposure accidents. The experiment showed that the culture time should be prolonged properly after high dose exposure, and no significant differences were observed between 52—72h culture. The dose-response curve of 6—22Gy fitted to linear-square model ,Y,=-2.269+0.776D-7.868 ×10,-3,D,2, and is reliable through verification of the accident dose estimations. In this study, the dose-response curve and equation of chromosome dic+r after 6—22Gy high dose irradiation were established firstly, and exact dose estimation can be achieved according to it.

关键词

特大剂量γ射线染色体畸变剂量效应曲线事故剂量估算

Keywords

Very high dose γ-rayChromosome aberrationDose-response curveAccident dose estimation

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