Mechanisms of bone metabolism abnormality in rats induced by γ-ray irradiation of the kidneys

GAO Linfeng; WANG Hongfu

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Mechanisms of bone metabolism abnormality in rats induced by γ-ray irradiation of the kidneys

更新时间：2023-03-30

- Mechanisms of bone metabolism abnormality in rats induced by γ-ray irradiation of the kidneys
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 5, Pages: 303-307(2006)
- 作者机构：
  
  1.上海市疾病预防控制中心　上海　200336
  2.复旦大学放射医学研究所　上海　200032
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GAO Linfeng, WANG Hongfu. Mechanisms of bone metabolism abnormality in rats induced by γ-ray irradiation of the kidneys. [J]. Journal of Radiation Research and Radiation Processing 24(5):303-307(2006)
DOI：

GAO Linfeng, WANG Hongfu. Mechanisms of bone metabolism abnormality in rats induced by γ-ray irradiation of the kidneys. [J]. Journal of Radiation Research and Radiation Processing 24(5):303-307(2006) DOI：

摘要

研究了大剂量γ射线照射大鼠肾脏后诱发骨代谢异常的细胞分子机制。以15Gy γ射线照射大鼠肾脏，3个月后分析骨骼的病理形态、相关基因mRNA和蛋白质表达量的改变。肾辐射致骨小梁体积、骨小梁宽度、骨小梁数目分别降低13.2%、18.3%(,p,<,0.05)和20.1%( ,p,<,0.05)，骨小梁间距增加34.8%(,p,<,0.05)，骨吸收表面提高20.2%(,p,<,0.05)。骨骼RANKL/OPG（细胞核因子κB受体活化因子配基，Receptor activator of nuclear factor-kappaB ligand, RANKL; 骨保护蛋白, Osteoprotegerin, OPG）mRNA比值提高52.2%(,p,<,0.05)。TGF-β（转移生长因子β, Transforming growth factor-β）、IGF-I（胰岛素样生长因子I, Insulin-like growth factor I,）、OPN（骨桥蛋白, Osteopontin）mRNA和蛋白质表达量明显升高。结果表明：大剂量γ射线照射大鼠肾脏可导致明显骨代谢异常，其发生机制主要与肾1线羟化酶活性降低致活性维生素D减少及PTH升高，成骨细胞表达的RANKL/OPG比例失调，以及TGF-，、IGF-I、OPN表达明显升高有关。

Abstract

A study was done on cellular molecule mechanisms of abnormality of bone metabolism induced by 15Gy γ-ray exposure to rat kidneys. Three months after the kidney irradiation, the rats were sacrificed for analyzing changes in bone pathological morphous and expressions of related genes and proteins. In the irradiated group, TV/BV, Tb.Th, Tb.N decreased by 13.2%, 18.3%(,p,<,0.05) and 20.1%(,p,<,0.05), respectively, Tb.Sp increased by 34.8% (,p,<,0.05), ES/BS increased by 20.2%(,p,<,0.05). The ratio of RANKL/OPG mRNA expression increased by 52.2% (,p,<,0.05). The expressions of mRNA and proteins of TGF-β, IGF-I, and OPN in the bone increased significantly. In conclusion, the 15Gy γ-ray irradiation of rat kidneys could result in significant abnormality of bone metabolism. Mechanisms of abnormality of bone metabolism induced by kidney exposure to γ-rays could be impairment of alpha-hydroxylase in the kidney, which leads to insufficient of active Vit-D and increased serum PTH, worsened imbalance of the ratio of RANKL/OPG, and significant increased TGF-β, IGF-I and OPN in osteoblasts.

关键词

电离辐射肾脏骨代谢机制

Keywords

Ionizing radiationKidneyBone metabolismMechanism

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