Mutation effects of kojic acid production strain induced by synchrotron radiation of soft X-rays and kinetics of the fermentation

CHEN Liang; JIANG Shiping; WAN Libiao; MA Xiaodong; LI Meifang

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Mutation effects of kojic acid production strain induced by synchrotron radiation of soft X-rays and kinetics of the fermentation

更新时间：2023-03-30

- Mutation effects of kojic acid production strain induced by synchrotron radiation of soft X-rays and kinetics of the fermentation
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 5, Pages: 308-312(2006)
- 作者机构：
  
  1.中国科学技术大学国家同步辐射实验室　合肥　230029
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CHEN Liang, JIANG Shiping, WAN Libiao, et al. Mutation effects of kojic acid production strain induced by synchrotron radiation of soft X-rays and kinetics of the fermentation. [J]. Journal of Radiation Research and Radiation Processing 24(5):308-312(2006)
DOI：

CHEN Liang, JIANG Shiping, WAN Libiao, et al. Mutation effects of kojic acid production strain induced by synchrotron radiation of soft X-rays and kinetics of the fermentation. [J]. Journal of Radiation Research and Radiation Processing 24(5):308-312(2006) DOI：

摘要

选择能量在氧的K吸收边附近的同步辐射软X射线对米曲霉孢子的辐照致死效应进行了研究。通过筛选，获得一株曲酸高产突变株。500mL摇瓶10d的发酵表明，突变株的累积产酸量（27.79g/L）较原发菌株的（17.81g/L）提高约56％。同时，改变发酵液中不同氮源和碳源，对突变株的发酵条件进行了比较研究。实验结果表明，同步辐射软X射线是进行曲酸生产菌诱变选育的潜在诱变源。

Abstract

The irradiation effect on ,Aspergillus oryzae, spores was studied by 0.54keV X-rays (about the K shell absorption edge of oxygen) from the synchrotron facility at NSRL. A high production mutant for kojic acid was obtained from the spores irradiated by soft X-rays, which accumulated 27.79g kojic acid per L in 500mL shake-flask fermentation for 10 days using glucose as carbon source, and has increased about 56% of production than that of the original (17.81g/L). Also the fermentation conditions were studied with different carbon sources and nitrogen sources. The results showed that the mutant induced by the X-rays of synchrotron radiation would be of potential for high kojic acid production.

关键词

米曲霉同步辐射软X射线曲酸

Keywords

Aspergillus oryzaeSynchrotron radiationSoft X-raysKojic acid

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1(Wenzhou Medical University,,)

2(Jiading campus, Shanghai Institute of Applied Physics, Chinese Academy of Sciences,,)

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