Estimation of genetic effects of static magnetic fields (SMFs) on the pollen mother cells (PMCs) of wheats
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Estimation of genetic effects of static magnetic fields (SMFs) on the pollen mother cells (PMCs) of wheats
Journal of Radiation Research and Radiation ProcessingVol. 25, Issue 4, Pages: 225-231(2007)
作者机构:
1.中国科学院等离子体物理研究所离子束生物工程重点实验室 合肥 230031
2.安徽大学生命科学学院生态工程与生物技术安徽省重点实验室 合肥 230039
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ZHANG Pingping, YIN Ruochun, TANG Mingli, et al. Estimation of genetic effects of static magnetic fields (SMFs) on the pollen mother cells (PMCs) of wheats. [J]. Journal of Radiation Research and Radiation Processing 25(4):225-231(2007)
DOI:
ZHANG Pingping, YIN Ruochun, TANG Mingli, et al. Estimation of genetic effects of static magnetic fields (SMFs) on the pollen mother cells (PMCs) of wheats. [J]. Journal of Radiation Research and Radiation Processing 25(4):225-231(2007)DOI:
Estimation of genetic effects of static magnetic fields (SMFs) on the pollen mother cells (PMCs) of wheats
研究了静态强磁场(Static magnetic fields, SMFs)对小麦花粉母细胞的影响,以评价环境中这种非电离辐射可能的遗传毒性。将小麦种子暴露于不同磁场强度(1、3、5、7T)/5h及进行不同时间(1、3、5h)/7T的处理,取其幼穗进行常规制片。以微核、染色体桥、落后染色体、断片、非四极分裂等的频率作为DNA损伤的指标来评价SMFs的遗传毒性作用。结果表明,5T以下剂量,暴露组与对照组之间在畸变频率上无统计学意义上的差别,5T场强下染色体桥、7T场强下落后染色体、三极分离、微核的频率则有显著的提高。剂量-效应呈现出剂量依赖的非线性关系,表明5T以上静态强磁场可能对生物体具有某种遗传损伤效应。
Abstract
The effect of static magnetic fields (SMFs) on the chromosome aberrations in the pollen mother cells (PMCs) of wheat was studied. The seeds of wheat were exposed to static magnetic fields of different magnetic flux density (0, 1, 3, 5 and 7 Tesla) for 5h and with different treated time (1, 3 and 5h) at magnetic flux density of 7 Tesla. The genotoxic effect was evaluated in terms of micronucleus (MN), chromosomal bridge, lagging chromosome, abnormal segregation and fragment in PMCs. The results indicated that the exposed groups of low field intensity (below 5 Tesla) showed no statistically significant difference in aberration frequency compared with unexposed control groups and sham exposed groups, but significant differences of chromosomal bridge at 5T and lagging chromosome, triple-polar segregation or micronucleus at 7T were detected in the exposed group (,p,<,0.05). The dose-effect relationships indicated that the increase frequency of meiotic abnormal cells correlated with flux density of magnetic field and treated time, but no linear effect was observed. These results lead us to the conclusion that high static magnetic fields above 5T for 5h duration might cause an increase in potential damage to organisms.
关键词
染色体畸变微核静态磁场花粉母细胞
Keywords
Chromosomal aberrationMicronucleusStatic magnetic fieldsPollen mother cells
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