Breeding of welan gum-producing bacteria by low energy N<sup>+</sup> ion implantation

GUO Chaojiang; QIAO Hongqun; LI Sha; LI Hui; XU Hong

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Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation

更新时间：2023-03-30

- Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 5, Pages: 266-270(2007)
- 作者机构：
  
  1.南京工业大学制药与生命科学学院　南京　210009
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GUO Chaojiang, QIAO Hongqun, LI Sha, et al. Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation. [J]. Journal of Radiation Research and Radiation Processing 25(5):266-270(2007)
DOI：

GUO Chaojiang, QIAO Hongqun, LI Sha, et al. Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation. [J]. Journal of Radiation Research and Radiation Processing 25(5):266-270(2007) DOI：

摘要

利用低能N,+,束对威兰胶生产菌进行了辐射诱变选育研究。能量为20keV，注入剂量为20×10,14,/cm,-2,时，筛选出一株高产菌株,Alcaligenes, sp.NX-3-1。结果表明，摇瓶发酵中诱变菌威兰胶产量比出发菌高27.5%；在7.5L发酵罐上，,Alcaligenes, sp.NX-3-1菌生长速度比出发菌快，生物量高，底物葡萄糖消耗量多，威兰胶产量较出发菌有明显提高，最高达26.4g •L,-1,。

Abstract

Breeding of welan gum-producing bacteria by low energy N,+, ion implantation has been investigated. A high production mutant NX-3-1 for welan gum was screened under the implantation conditions of 20keV, 20×10,14, /cm,-2, the average production of welan gum with NX-3-1 has been raised by 27.5% compared to the original strain in shaking flask culture. NX-3-1 grew much faster than original strain companied with higher glucose consumption in 7.5L fermenter. The maximum welan gum concentration with NX-3-1 was 26.4 g • L,-1,.

关键词

离子注入诱变选育威兰胶发酵

Keywords

Ion implantationWelan gumMutation breedingFermentation

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Related Institution

Ion Beam Bio-engineering Center, Xinjiang University

Food Science and Technology College, Nanjing Agricultural University

Longping Branch, Graduate School of Hunan University

Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences/ Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation

Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, Institute of Advanced Materials Physics

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Breeding of welan gum-producing bacteria by low energy N+ ion implantation

DOI：

摘要

Abstract

关键词

Keywords

references

Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation