The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization

XING Chungen; LI Guoqiang; ZHOU Liying; JIANG Yinfen; LIU Yulong; XUE Yongquan; LU Xueguan; WU Yafang; DAI Hong; TIAN Ye

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The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization

更新时间：2023-03-30

- The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization
- Journal of Radiation Research and Radiation Processing Vol. 25, Issue 5, Pages: 308-311(2007)
- 作者机构：
  
  1.苏州大学附属第二医院　苏州　215004
  2.苏州大学附属第一医院　苏州　215006
- 作者简介：
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XING Chungen, LI Guoqiang, ZHOU Liying, et al. The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization. [J]. Journal of Radiation Research and Radiation Processing 25(5):308-311(2007)
DOI：

XING Chungen, LI Guoqiang, ZHOU Liying, et al. The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization. [J]. Journal of Radiation Research and Radiation Processing 25(5):308-311(2007) DOI：

摘要

探讨荧光原位杂交技术预测人大肠癌细胞内在放射敏感性的可行性。应用荧光原位杂交技术检测人大肠癌动物模型照射后癌细胞2号染色体易位畸变并分析其与照射后肿瘤生长曲线的相关性。结果表明，放疗使Lovo移植瘤的增长明显延缓，Lovo细胞的放射敏感性高于SW480细胞。X射线照射后肿瘤荧光原位杂交技术检测结果显示：Lovo细胞的2号染色体诱导易位畸变量显著大于SW480细胞（,p,<,0.05）。荧光原位杂交技术能快捷、可靠地预测人大肠癌细胞的放射敏感性，从而为临床大肠癌病人放疗的选择提供重要参考依据。

Abstract

To study the correlation between the number of radiation-induced translocations of chromosome 2 and the cell survival fraction after irradiation using fluorescence in situ hybridization , and discuss the feasibility to predict the radiosensitivity of human colorectal cancer cell using FISH. The result shows that The growth of implanted tumor had been restrained after irradiation. The sensitivity of Lovo cell was higher than SW480. The result of FISH detection to implanted tumor resected indicated that the number of chromosome 2 aberration of Lovo cell was higher than SW480 significantly (,p,<,0.05). Fluorescence in Situ Hybridization can be used to predict the intrinsic radiosensitivity of human colorectal cancer cell conveniently and reliably. It provides important references for the selection of radiotherapy in the patients suffering colorectal cancer clinically.

关键词

荧光原位杂交预测大肠癌细胞放射敏感性

Keywords

Fluorescence in situHybridizationPredictionColorectal cancerRadiosensitivity

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Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology

Department of Cell Biology, School of Medicine, Anhui University of Science and Technology

First Clinical Hospital, School of Medicine, Anhui University of Science and Technology

Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University

苏州大学医学部放射医学与防护学院/放射医学及交叉学科研究院

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