Mutagenic effects of microwave irradiation on high yield L-lattic acid producingstrain Lactobacillus casei
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Mutagenic effects of microwave irradiation on high yield L-lattic acid producingstrain Lactobacillus casei
Journal of Radiation Research and Radiation ProcessingVol. 25, Issue 5, Pages: 316-320(2007)
作者机构:
1.哈尔滨工业大学市政环境工程学院 哈尔滨 150090
2.哈尔滨商业大学食品科学与工程学院 哈尔滨 150076
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XU Wei, WANG Peng, ZHANG Xing. Mutagenic effects of microwave irradiation on high yield L-lattic acid producingstrain Lactobacillus casei. [J]. Journal of Radiation Research and Radiation Processing 25(5):316-320(2007)
DOI:
XU Wei, WANG Peng, ZHANG Xing. Mutagenic effects of microwave irradiation on high yield L-lattic acid producingstrain Lactobacillus casei. [J]. Journal of Radiation Research and Radiation Processing 25(5):316-320(2007)DOI:
Mutagenic effects of microwave irradiation on high yield L-lattic acid producingstrain Lactobacillus casei
在低功率微波条件下,并采用水循环(20℃)冷却装置,对干酪乳杆菌鼠李糖亚种(,Lactobacillus casei subsp. rhamnosus,)CICC 6013进行诱变处理,通过对微波辐照功率和微波辐照时间做单因素平行实验,得到最高产酸量为124.15g/L的突变株W4-23,比原始菌株产酸发酵量提高了53.45%,连续遗传8代,产酸性状稳定。研究结果表明,最佳诱变条件为在400W微波功率下辐照20min。
Abstract
This paper focused on the mutagenicity of microwave irradiation with low-power on ,Lactobacillus casei subsp. rhamnosus, 6013 cooled with circulating water of 20℃. In the parallel experiments by adjusting the power and irradiation time, a mutant strain W4-23 with L-lattic acid production of 124.15g/L higher than 80g/L of the parent strain had been obtained. And the properties of W4-23 with highly producing L-attic acid remained stable after cultured for 8 times and stored for two months at 4℃. It was concluded that the mutagencity could be stimulated under the microwave power of 400W and irradiation length of 20min.
关键词
微波辐照诱变效应干酪乳杆菌L-乳酸
Keywords
Microwave irradiationMutagenic effectsL-attic acidLactobacillus casei
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1 (Institute of Modern Physics, Chinese Academy of Sciences,,)