Adaptive response of apoptosis in EL-4 lymphoma cells induced by low dose radiation and its mechanism

LIU Shuchun; GONG Pingsheng; WANG Zhicheng; SUN Liguang; GONG Shouliang

您当前的位置：

首页 >

文章列表页 >

Adaptive response of apoptosis in EL-4 lymphoma cells induced by low dose radiation and its mechanism

更新时间：2023-03-30

- Adaptive response of apoptosis in EL-4 lymphoma cells induced by low dose radiation and its mechanism
- Journal of Radiation Research and Radiation Processing Vol. 26, Issue 2, Pages: 70-73(2008)
- 作者机构：
  
  1.吉林大学公共卫生学院卫生部放射生物学重点实验室　长春　130021
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
LIU Shuchun, GONG Pingsheng, WANG Zhicheng, et al. Adaptive response of apoptosis in EL-4 lymphoma cells induced by low dose radiation and its mechanism. [J]. Journal of Radiation Research and Radiation Processing 26(2):70-73(2008)
DOI：

LIU Shuchun, GONG Pingsheng, WANG Zhicheng, et al. Adaptive response of apoptosis in EL-4 lymphoma cells induced by low dose radiation and its mechanism. [J]. Journal of Radiation Research and Radiation Processing 26(2):70-73(2008) DOI：

摘要

用X射线照射离体EL-4淋巴瘤细胞，其诱导剂量（D1）为25 ~ 200 mGy（剂量率12.5 mGy/min），攻击剂量（D2）为1.5 Gy（剂量率287 mGy/min），D1和D2间隔6 h。通过流式细胞仪检测其细胞凋亡百分数及其相关基因蛋白表达水平。其结果显示，当D1为25 ~ 100 mGy，D2为1.5 Gy；或D1为75 mGy，D2为0.5 ~ 2.0 Gy，与D2组比较，D1 + D2组细胞凋亡百分数有显著意义降低。当D1为25 ~ 100 mGy，D2为1.5 Gy，与D2组比较，D1 + D2组Bcl-2蛋白阳性百分数不同程度增加，Bax蛋白有显著意义降低，Bcl-2/Bax比值有显著意义增高；p53蛋白不同程度降低。其结果提示，在D1为25 ~ 100 mGy，D2为0.5 ~ 2.0 Gy，可诱导EL-4淋巴瘤细胞凋亡的适应性反应，其相关凋亡基因蛋白Bcl-2、Bax和p53发生的改变，与其细胞凋亡明显相关，可能在其适应性反应的机制中起到重要的调节作用。

Abstract

EL-4 lymphoma cells were irradiated with the inductive doses (D1: 25 ~ 200 mGy, dose rate: 12.5mGy/min) and the challenging dose (D2: 0.5 ~ 3.0 Gy, dose rate: 287 mGy/min), and the time interval between D1 and D2 was 6 h. The percentage of cell apoptosis and the expressive levels of cell apoptosis-associated gene proteins were measured with flow cytometry. The percentages of cell apoptosis in the D1 + D2 group with 25 ~ 100 mGy (D1) and 1.5 Gy (D2) or 75 mGy (D1) and 25 ~ 200 mGy (D2) were significantly lower than those in the D2 group. As compared with the D2 group, the positive percentage of cell Bcl-2 protein expression increased somewhat, and Bax decreased significantly, meanwhile the ratio of Bcl-2/Bax increased significantly and p53 decreased somewhat in D1 + D2 group with 25 ~ 100 mGy (D1) and 1.5 Gy (D2). The adaptive response of EL-4 lymphoma cell apoptosis could be induced by pre-irradiation with 25 ~ 100 mGy. Meantime, the expressive levels of cell apoptosis-associated gene Bcl-2, Bax and p53 proteins could change accordingly with cell apoptosis. These gene protein changes may play an important role in the mechanism of the adaptive response.

关键词

电离辐射淋巴瘤细胞细胞凋亡凋亡基因抗凋亡基因适应性反应

Keywords

Ionizing radiationLymphoma cellCell apoptosisApoptotic geneAnti-apoptotic geneAdaptive response

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

STUDY ON EFFECT OF CD28 AND Fas IN CELL APPOPTOSIS OF INIZING RADIATION INDUCED

CROSS-ADAPTIVE RESPONSE BETWEEN IONIZING RADIATION AND CADMIUN

miR-34a在辐射损伤效应中的作用

Preparation of hydrogel-based photocatalytic composite materials by radiation technology

Clustered DNA damages induced by low-energy (1~20 eV) electrons and the general mechanism of transient anions

Related Author

No data

Related Institution

College Nuclear Medicine Suzhou University

Department of Toxicology, BeiJing Medical University

(School of Radiation Medicine and Protection, Medical College of Soochow University,,)

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Hubei University of Science and Technology

⁰