Study on breeding of daptomycin-producing strains by nitrogen ion implantation

ZHOU Jian; LIU Ying; FANG Dongsheng; JIANG Hong; ZHANG Yin; GAO Wuyan

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Study on breeding of daptomycin-producing strains by nitrogen ion implantation

更新时间：2023-03-30

- Study on breeding of daptomycin-producing strains by nitrogen ion implantation
- Journal of Radiation Research and Radiation Processing Vol. 26, Issue 5, Pages: 317-320(2008)
- 作者机构：
  
  1.福建省微生物研究所　福建省新药（微生物）筛选重点实验室　福州　350007
- 作者简介：
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ZHOU Jian, LIU Ying, FANG Dongsheng, et al. Study on breeding of daptomycin-producing strains by nitrogen ion implantation. [J]. Journal of Radiation Research and Radiation Processing 26(5):317-320(2008)
DOI：

ZHOU Jian, LIU Ying, FANG Dongsheng, et al. Study on breeding of daptomycin-producing strains by nitrogen ion implantation. [J]. Journal of Radiation Research and Radiation Processing 26(5):317-320(2008) DOI：

摘要

通过离子注入技术对达托霉素产生菌玫瑰孢链霉菌（,Streptomyces roseosporus,）进行诱变选育，使用N,+,作为离子源，初步探讨N,+,注入对达托霉素生产菌所产生的生物学效应。通过链霉素抗性筛选法获得多株遗传稳定性较好的高产突变株，其中突变株N3-36发酵单位比出发菌株提高了26%。

Abstract

Streptomyces roseosporus, C20, the bacteria used in production of daptomycin, were implanted with (15~200)×10,13,/cm,2, of 20keV N,+,ions. Survival rate of the bacteria at different absorbed doses was investigated, and mutagenic effects of the microbe were studied. After breeding under the selection pressure of resistance to streptomycin (the lethal concentration is 1.2μg/mL), several mutant strains with higher yields of daptomycin have been obtained. One of mutant strains, N3-36, can increase up to 126% compared to the original strain. It also shows that the mutant strains have high genetic stability.

关键词

达托霉素离子注入链霉素抗性筛选

Keywords

DapotomycinIon implantationScreening of streptomycin-resistance

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