Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation

ZHOU Liwei; CHEN Xiaoming; ZHANG Jianguo; LIU Fang

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Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation

更新时间：2023-03-30

- Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation
- Journal of Radiation Research and Radiation Processing Vol. 26, Issue 6, Pages: 321-325(2008)
- 作者机构：
  
  1.西南科技大学生命科学与工程学院　绵阳　621010
- 作者简介：
- 基金信息：
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ZHOU Liwei, CHEN Xiaoming, ZHANG Jianguo, et al. Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation. [J]. Journal of Radiation Research and Radiation Processing 26(6):321-325(2008)
DOI：

ZHOU Liwei, CHEN Xiaoming, ZHANG Jianguo, et al. Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation. [J]. Journal of Radiation Research and Radiation Processing 26(6):321-325(2008) DOI：

摘要

DNA既是生命物质中信息的载体，也是辐射生物效应最主要的靶分子。电离辐射通过射线的直接作用和间接作用引起DNA分子的多类型损伤，其中DNA双链断裂（Double-strand breaks，DSB）是辐射引起的各种生物效应中最重要的原初损伤之一，成为辐射生物学研究的重点。目前 DSB研究的主要方法包括拉曼光谱技术、原子力显微镜、单细胞凝胶电泳、脉冲场凝胶电泳、γ H2AX分析技术、早熟染色体凝集等，研究DSB的统计模型包括随机断裂模型、Moment法、Tsallis熵模型、平均分子量法，在此均得到总结，最后探讨了DSB辐照热点的研究前景。

Abstract

A DNA molecule is both the carrier of biological information and the main target of biological effects induced by ionizing radiation. Irradiation leads to many types of DNA damage by the direct and the indirect radiation effect. DNA double-strand breaks (DSB) induced by ionizing radiation represent the primary damage of all bio-effects. Currently, the detecting methods dealing with DSB include raman spectroscopy analysis, atomic force microscopy analysis, single cell gel electrophoresis, pulsed-field gel electrophoresis, γ H2AX analysis and premature chromosome condensation analysis, etc. And the statistical models of DNA DSB involve in the random fracture model, the moment method, the tsallis entropy model and the average molecular weight method. Both of them have been discussed in this paper. Finally, the research prospect of the DNA DSB radiation hotspot is presented.

关键词

DNA双链断裂双链断裂研究方法双链断裂统计模型双链断裂辐照热点

Keywords

DNA double-strand breaksDouble-strand breaks detect methodsDouble-strand breaks statistical modelsDouble-strand breaks radiation hotspot

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