Selection of <italic style="font-style: italic">Trichoderma viride strains</italic> irritated by laser and optimization of the xylanase fermentation

LIU Zhenzhen; MAO Wenjun; ZHU Hong; ZHU Rongrong; SUN Xiaoyu; YAO Side; WANG Shilong

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Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation

更新时间：2023-03-30

- Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation
- Journal of Radiation Research and Radiation Processing Vol. 26, Issue 6, Pages: 335-342(2008)
- 作者机构：
  
  1.同济大学生命科学与技术学院　上海　200092
  2.中国科学院上海应用物理研究所　上海　201800
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LIU Zhenzhen, MAO Wenjun, ZHU Hong, et al. Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation. [J]. Journal of Radiation Research and Radiation Processing 26(6):335-342(2008)
DOI：

LIU Zhenzhen, MAO Wenjun, ZHU Hong, et al. Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation. [J]. Journal of Radiation Research and Radiation Processing 26(6):335-342(2008) DOI：

摘要

本实验利用激光这一高效诱变手段，对野生菌株绿色木霉Tr-02进行辐照诱变，通过两轮的定向筛选，最终得到一株高产木聚糖酶的菌株。对突变株进行发酵条件优化，结果表明，以0.5%麸皮为碳源，0.5%蛋白胨为氮源，0.1%Tween-80作为产酶促进剂，培养基初始pH值为5.0，瓶装量100mL，接种两环，28℃，180 r/min培养48h，木聚糖酶活力达到281.21U·mL,-1,，较之相同条件出发菌株木聚糖酶活力增长31.83%。

Abstract

A mutation of wild strain ,Trichoderma Viride, Tr-02 irradiated by laser was studied in this paper. A high xylanase-producing strain was obtained by two steps oriental screening finally. The results show that an optimization condition of the xylanase fermentation is as following: 0.5% corncob as the carbon source, 0.5% peptone as the nitrogen source, 0.1% Tween-80 as a enzyme-promoting agent, initial medium pH to 5.0, medium volume to 100mL, vaccinated two rings of spores, 28℃, 180 r/min, fermented 48h. The xylanase activity of the mutant reaches 281.21U/mL and is 31.83% higher than that of the original strain under the same conditions.

关键词

激光绿色木霉木聚糖酶发酵

Keywords

LaserTrichoderma virideXylanaseFermentation

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Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences/ Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation

Longping Branch, Graduate School of Hunan University

Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology

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Ion Beam Bio-engineering Center, Xinjiang University

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Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation

DOI：

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