Increased expression of UHRF1 reduces radiosensitivity of breast cancer MDA-MB-231 cells

LI Xinli; FAN Saijun

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Increased expression of UHRF1 reduces radiosensitivity of breast cancer MDA-MB-231 cells

更新时间：2023-03-30

- Increased expression of UHRF1 reduces radiosensitivity of breast cancer MDA-MB-231 cells
- Journal of Radiation Research and Radiation Processing Vol. 26, Issue 6, Pages: 357-362(2008)
- 作者机构：
  
  1.苏州大学医学部放射医学与公共卫生学院　苏州　215123
  2.美国华盛顿特区乔治敦大学Lombardi肿瘤研究中心
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LI Xinli, FAN Saijun. Increased expression of UHRF1 reduces radiosensitivity of breast cancer MDA-MB-231 cells. [J]. Journal of Radiation Research and Radiation Processing 26(6):357-362(2008)
DOI：

LI Xinli, FAN Saijun. Increased expression of UHRF1 reduces radiosensitivity of breast cancer MDA-MB-231 cells. [J]. Journal of Radiation Research and Radiation Processing 26(6):357-362(2008) DOI：

摘要

为了了解基因UHRF1（ubiquitin-like, containing PHD and RING finger domains, 1）基因对乳癌细胞的生长、增殖和辐射敏感性的影响，将全长UHRF1基因的cDNA克隆至真核表达载体pcDNA3中，通过脂质体介导转染入乳癌细胞MDA-MB-231，筛选出UHRF1高表达的细胞株。借助生长曲线、集落形成实验，研究基因转染细胞的生长、增殖能力；通过γ射线辐照后的集落形成实验，观察基因转染对细胞辐射敏感性的影响。实验结果显示，与MDA-MB-231母细胞和转染了pcDNA3“空”质粒的MDA-MB-231/pcDNA3细胞相比，转染有UHRF1基因的MDA-MB-231/UHRF1细胞的生长率和克隆形成率都未有明显改变，但细胞的辐射敏感性明显降低，这些结果首次揭示人类UHRF1基因的表达水平并不影响乳癌细胞MDA-MB-231的生长和增殖能力，但可以调节其辐射敏感性，具体机制有待进一步深入研究。

Abstract

In order to study the effect of UHRF1 on growth and radiosensitivity of breast cancer MDA-MB-231 cells, The stable clones of MAD-MB-231 cells with UHRF1 gene overexpression were established by transfection of a full-length human UHRF1 cDNA which was cloned into a mammalian expression vector pcDNA3. And the effect of UHRF1 on growth of MDA-MB-231 cells was identified by population double time and colony formation assays. The change of radiosensitivity after γ-irradiation was determined by colony formation assay. It has been found that enhanced expression of UHRF1 does not affect cell growth and colony formation. However, in comparison with MAD-MB-231 parental cells and MAD-MB-231/pcDNA3 cells, MAD-MB-231/UHRF1 cells are less susceptibility to γ-irradiation. These results indicate, for the first time, that human UHRF1 may be an important mediator in radiotherapy of breast cancer, although further study is still needed.

关键词

人类UHRF1基因乳癌稳定转染细胞生长辐射敏感性

Keywords

Human UHRF1 geneBreast cancerStable transfectionCell growthRadiation sensitivity

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