Reasonable threshold value used to segment the individual comet from the comet assay image

YAN Xuekun; CHEN Ying; DU Jie; ZHANG Xueqing; LUO Yisheng

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Reasonable threshold value used to segment the individual comet from the comet assay image

更新时间：2023-03-30

- Reasonable threshold value used to segment the individual comet from the comet assay image
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 1, Pages: 15-19(2009)
- 作者机构：
  
  1.军事医学科学院　放射与辐射医学研究所　北京　100850
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YAN Xuekun, CHEN Ying, DU Jie, et al. Reasonable threshold value used to segment the individual comet from the comet assay image. [J]. Journal of Radiation Research and Radiation Processing 27(1):15-19(2009)
DOI：

YAN Xuekun, CHEN Ying, DU Jie, et al. Reasonable threshold value used to segment the individual comet from the comet assay image. [J]. Journal of Radiation Research and Radiation Processing 27(1):15-19(2009) DOI：

摘要

彗星实验（单细胞凝胶电泳）图像自动分析中，彗星轮廓的合理分割是后续各项指标分析的前提。首先采用几种常用的图像边缘检测算法对彗星轮廓进行提取，并分析了彗星图像的特点；然后对文献中提到的和现有彗星分析软件（如CASP、TriTek CometScoreTM）中的彗星轮廓分割方法进行比较；最后采用一种基于图像内容的阈值分割方法，实现了最佳阈值的选择，并得出了彗星轮廓分割的几点原则。

Abstract

Reasonable segmentation of the individual comet contour from the Comet Assay (CA) images is the precondition for all of parameters analysis during the automatic analyzing for the CA. The Otsu method and several arithmetic operators for image segmentation, such as Sobel, Prewitt, Roberts and Canny were used to segment the comet contour, and characters of the CA images were analyzed firstly. And then the segmentation methods which had been adopted in the software for CA automatic analysis, such as the CASP, the TriTek CometScore,TM, were put forward and compared. At last, a two-step procedure for threshold calculation based on image-content analysis is adopted to segment the individual comet from the CA images, and several principles for the segmentation are put forward too.

关键词

彗星实验图像分析分割阈值剂量－效应曲线

Keywords

Comet assayImage analysisSegmentation thresholdDose-responses curve

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