Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A

LIN Xiaoli; FANG Huasheng; ZHANG Fengqiu; YA Huiyuan; QIN Guangyong

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Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A

更新时间：2023-03-30

- Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 1, Pages: 10-14(2009)
- 作者机构：
  
  1.郑州大学离子束生物工程省重点实验室　郑州　450052
  2.郑州大学物理工程学院　郑州　450052
  3.洛阳师范学院生命科学系　洛阳　471022
- 作者简介：
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LIN Xiaoli, FANG Huasheng, ZHANG Fengqiu, et al. Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A. [J]. Journal of Radiation Research and Radiation Processing 27(1):10-14(2009)
DOI：

LIN Xiaoli, FANG Huasheng, ZHANG Fengqiu, et al. Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A. [J]. Journal of Radiation Research and Radiation Processing 27(1):10-14(2009) DOI：

摘要

利用流式细胞仪及荧光定量PCR技术，分别检测细胞凋亡率和DNA损伤修复相关基因mRNA表达变化，以探讨曲古菌素A（Trichostatin A, TSA）能否在体外增强食管癌细胞对X射线的敏感性。结果显示，辐射前TSA的处理显著提高了X射线引起的细胞凋亡率（,P,<,0.05）；X射线的单独处理显著上调了基因,ATM、XRCC2, 和,Lig4,(8Gy除外)的mRNA水平（,P,<,0.05），TSA单独和TSA联合X射线都下调了,ATM、XRCC2,和,Lig4,的mRNA水平。提示TSA很可能通过下调,ATM,、,Lig4,和,XRCC2, mRNA水平使食管癌细胞对X射线敏感。

Abstract

Cell apoptosis and the expression of DNA damage repair-related genes ,XRCC2,ATM, and ,Lig4, were detected separately by flow cytometry analysis and real-time fluorescence quantitative (RT-PCR), in order to investigate the effect of Trichostatin A(TSA)pre-treatment on the radiosensitivity of esophageal cancer EC9706 cells to X ray in vitro. The results indicate that pre-irradiation exposure to TSA enhances cell apoptotic rate by X ray markedly (,P,<,0.05) and the mRNA levels of ,XRCC2,ATM, and ,Lig4,(except 8Gy)are up-regulated by X ray individually(,P,<,0.05). TSA alone and TSA in combination with X ray all reduce mRNA levels of ,XRCC2,ATM, and ,Lig4,. In conclusions, TSA may radio-sensitize EC9706 cells to X ray by reducing mRNA levels of ,XRCC2,ATM, and ,Lig4,.

关键词

曲古菌素A食管癌细胞X射线辐射敏感性凋亡

Keywords

Trichostatin A (TSA)Esophagealcancer cellX rayRadio-sensitivityApoptosis

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