Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition

MAO Wenjun; LIU Zhenzhen; ZHU Hong; ZHU Rongrong; SUN Xiaoyu; YAO Side; WANG Shilong

您当前的位置：

首页 >

文章列表页 >

Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition

更新时间：2023-03-30

- Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 1, Pages: 1-4(2009)
- 作者机构：
  
  1.同济大学生命科学与技术学院　上海　200092
  2.中国科学院上海应用物理研究所　上海　201800
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
MAO Wenjun, LIU Zhenzhen, ZHU Hong, et al. Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition. [J]. Journal of Radiation Research and Radiation Processing 27(1):1-4(2009)
DOI：

MAO Wenjun, LIU Zhenzhen, ZHU Hong, et al. Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition. [J]. Journal of Radiation Research and Radiation Processing 27(1):1-4(2009) DOI：

摘要

本实验利用266 nm激光作为诱变手段，对酿酒酵母YE0进行辐照诱变筛选呼吸缺陷型酵母，通过TTC（2, 3, 5-氯化三苯基四氮唑)筛选和气相色谱法酒精测定，最终得到一株高葡萄糖转化率呼吸缺陷型酵母JB7。对该突变株进行鉴定和发酵条件优化，结果表明，接种量15%，发酵温度32℃，葡萄糖初始浓度25%，发酵72 h后，酒精产量体积分数可达12.3%，葡萄糖转化率达到52.9%，分别比出发菌株的酒精产量（12.0%）和葡萄糖转化率（49.6%）有所提高。高于正常酵母的糖原利用率，显示了呼吸缺陷型酵母良好的应用前景。

Abstract

A screen of respiration deficiency mutants of ,Saccharomyces Cerevisiae,YE0 induced by 266nm laser irradiation was studied. After the selective culture of TTC medium and determination of the alcohol yield by gas chromatographs, a respiration deficiency mutant JB7 with high glucose transformation efficiency was obtained. The results show that under the optimized fermentation conditions ( inoculation amount is 15%, glucose concentration is 25%, fermentation temperature is 32℃ and fermentation time is 72h) the alcoholic yield in volume fraction is 12.3% and the glucose transformation efficiency of JB7 is 52.9%, which are higher than that of the original strain YE0(glucose transformation efficiency is 49.6%, the alcoholic yield is 12.0%). The high glucose transformation efficiency shows a good prospect of application of the respiration deficiency yeast.

关键词

激光酿酒酵母发酵

Keywords

LaserSaccharomyces CerevisiaeFermentation

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation

Effects of γ-ray irradiation and particle size on enzymatic hydrolysis and fermentation of reed straws

Breeding of tacrolimus high-producing strains by gamma-ray radiation and nitrosoguanidine mutagent

Study of biological effects on Lentinula edodes by N⁺ ion implantation of low energy

Breeding of welan gum-producing bacteria by low energy N⁺ ion implantation

Related Author

No data

Related Institution

Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences/ Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation

Longping Branch, Graduate School of Hunan University

Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology

Quanzhou Medical College

Ion Beam Bio-engineering Center, Xinjiang University

⁰