Effect of MgSO<sub>4</sub> on the contents of Ca<sup>2+</sup> in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury

YUAN Wenjia; CUI Fengmei; WANG Lili; LIU Ping; HE Chao; TU Yu

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Effect of MgSO₄ on the contents of Ca²⁺ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury

更新时间：2023-03-30

- Effect of MgSO₄ on the contents of Ca²⁺ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 1, Pages: 53-56(2009)
- 作者机构：
  
  1.苏州大学医学部放射医学与公共卫生学院　苏州　215123
  2.苏州大学附属第一医院肿瘤放疗科　苏州　215006
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YUAN Wenjia, CUI Fengmei, WANG Lili, et al. Effect of MgSO₄ on the contents of Ca²⁺ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury. [J]. Journal of Radiation Research and Radiation Processing 27(1):53-56(2009)
DOI：

YUAN Wenjia, CUI Fengmei, WANG Lili, et al. Effect of MgSO₄ on the contents of Ca²⁺ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury. [J]. Journal of Radiation Research and Radiation Processing 27(1):53-56(2009) DOI：

摘要

为探讨硫酸镁（MgSO,4,）对电离辐射诱发脑损伤的保护作用，将成熟的SD大鼠36只随机分为空白对照组、实验对照组和硫酸镁实验用药组，用6 MeV电子束对实验组大鼠进行20 Gy全脑单次垂直照射，吸收剂量率为200 cGy/min；实验用药组大鼠于照射前1 d、照射后即刻和照射后连续5 d分别给予10%的MgSO,4,腹腔注射，分别于照后第3、10、17和24 d解剖大鼠，取其脑组织，用激光扫描共聚焦显微镜测定脑细胞内Ca,2+,的含量，硝酸还原酶法测定脑组织一氧化氮(NO)含量。结果显示，与空白对照组相比，实验对照组大鼠脑细胞内Ca,2+,平均荧光强度及脑组织中NO含量明显升高(,P,<,0.05)；与实验对照组相比，实验用药组大鼠脑细胞内Ca,2+,平均荧光强度及脑组织NO含量有所降低(,P,<,0.05)。表明早期使用硫酸镁可抑制辐射引起的脑细胞内钙离子的超载及脑组织中NO含量的升高，硫酸镁对放射性脑损伤有保护作用。

Abstract

The work is to explore the protection of magnesium sulfate(MgSO,4,) on radiation-induced acute brain injury. Thirty six mature Sprague-Dawley(SD) rats were randomly divided into 3 groups of control, experimental control and experimental therapy group. The whole brains of SD rats of experimental control and experimental therapy group were irradiated with a dose of 20 Gy using 6 MeV electron beam. MgSO,4, was injected into the abdomen of experimental therapy rats group 1day before, immediately and continue for 5days after irradiation respectively. The brain tissues were taken on 3, 10, 17 and 24 d after irradiation. Ca,2+, content in brain cell was measured by laser scanning confocal microscopy, and the NO content in brain tissue was detected by the method of nitric acid reductase. Compared with the blank control group, the contents of Ca,2+, in brain cell and NO in brain tissue of the experimental control group increase (,P,<,0.05). Compared with the experimental control group, the two indexes of the experimental therapy group decrease (,P,<,0.05). It shows that MgSO,4, used in early stage can inhibit the contents of Ca,2+, in brain cell and NO in brain tissue after radiation-induced acute brain injury. It means that MgSO,4, has a protective effect on radiation-induced acute brain injury.

关键词

放射性脑损伤硫酸镁细胞内钙离子一氧化氮

Keywords

Radiation-induced brain injuryMagnesium sulfateIntracellular free calciumNO

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College of Medicine, Henan University of Chinese Medicine

苏州大学医学部放射医学与防护学院

School of Radiation Medicine and Protection, Medical School of Soochow University

Shandong Academy of Occupational Health and Occupational Medicine

Department of Medical Radioprotection, School of Radiation Medicine and Public Health, Suzhou University

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Effect of MgSO4 on the contents of Ca2+ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury

DOI：

摘要

Abstract

关键词

Keywords

references

Effect of MgSO₄ on the contents of Ca²⁺ in brain cell and NO in brain tissue of rats with radiation-induced acute brain injury