Distribution of hydroxylapatite nanoparticles injected via intravenous in mouse

TANG Jinglong; LIU Guoquan; XIONG Ling; WANG Shuo; WANG Jianyu; LIU Li; LI Jiage; YUAN Fuqiang; XI Tingfei

您当前的位置：

首页 >

文章列表页 >

Distribution of hydroxylapatite nanoparticles injected via intravenous in mouse

更新时间：2023-03-30

- Distribution of hydroxylapatite nanoparticles injected via intravenous in mouse
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 2, Pages: 95-100(2009)
- 作者机构：
  
  1.北京科技大学材料学院　北京　100083
  2.中国药品生物制品检定所医疗器械监督检验中心　北京　100050
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
TANG Jinglong, LIU Guoquan, XIONG Ling, et al. Distribution of hydroxylapatite nanoparticles injected via intravenous in mouse. [J]. Journal of Radiation Research and Radiation Processing 27(2):95-100(2009)
DOI：

TANG Jinglong, LIU Guoquan, XIONG Ling, et al. Distribution of hydroxylapatite nanoparticles injected via intravenous in mouse. [J]. Journal of Radiation Research and Radiation Processing 27(2):95-100(2009) DOI：

摘要

研究了纳米羟基磷灰石（nano-HAP）颗粒静脉注射后在小鼠体内的分布、蓄积和排泄情况。将中子活化的nano-HAP尾静脉注射到小鼠体内，剂量为9 mg/kg。分别在15 min、1 h、2 h、4 h、8 h、24 h、72 h时断髓处死动物，解剖并收集动物的血液、脑、心、肝、脾、肺、肾、胃、小肠、胸骨、子宫或睾丸、粪便和尿液。用高氯酸和双氧水将收集的样品消解后，通过液体闪烁计数器测定各脏器组织和排泄物中的,45,Ca β射线计数来反映nano-HAP在动物体内的分布。结果显示，尾静脉注射nano-HAP后，大部分组织中,45,Ca β射线计数范围为0.5～250 Counts/min•mg，肺、肝、脾这3个部位中的,45,Ca β射线计数相当于其它脏器组织的6倍以上。整个试验期间，只有骨骼中的,45,Ca β射线计数变化不大，其它组织中的,45,Ca β射线计数均随时间延长而减少。各个时间点粪便中,45,Ca β射线计数要明显大于尿液中,45,Ca β射线计数。nano-HAP颗粒可以在体内大部分的器官和组织中分布，其中肺、肝、脾是nano-HAP分布最多的几个器官，而骨骼是nano-HAP唯一的蓄积器官。粪便排泄是体内nano-HAP排泄的主要途径。

Abstract

The present study was designed to investigate the distribution, accumulation and excretion of hydroxylapatite nanoparticles (nano-HAP) in mice with intravenous injection. The nano-HAP suspension was neutron activated and then stored 151d after neutron activation for experiment. The mice injected with radioactive nano-HAP heve sacrificed at 15min、1h、2h、4h、8h、24h、72h and the main organs were harvested for further measuring the counts of ,45,Ca in every sample by use of a liquid scintillation counter. Results indicate that ,45,Ca could be detected in all main organs (0.5~250Counts/min•mg), and there are more ,45,Ca exist in lung, liver and spleen. The counts of ,45,Ca in bone almost keep the same during the whole experiment period while counts of ,45,Ca of all other tissues decrease with time. And the counts of ,45,Ca exerted in faeces are higher than that in urine. So we can conclude that nano-HAP distribute in all organs of mice especially in lung, liver and spleen. But bone is the only tissue in which nano-HAP accumulated. Nano-HAP was excreted mostly through faeces.

关键词

纳米羟基磷灰石体内分布中子活化小鼠

Keywords

Hydroxylapatite nanoparticledistributionneutron activationmice

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

纳米羟基磷灰石颗粒肌肉注射后在小鼠体内的分布特性

Protective effects of a Chinese medicinal formula on the spleen of irradiated mice

Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice

Protection against γ ray-induced damage using herbal formulas

Protective effect of Lanzhou lily polysaccharide fragment on X-ray-induced injury of bone marrow nucleated cells in mice

Related Author

No data

Related Institution

（Institute for Medical Device Standardization Administration, and Institute for Medical Devices Control,National Institutes for Food and Drug Control,,）

Panzhihua Health Commission

Naval Medical Center of the PLA

Hubei University of Arts and Science, Xiangyang

Department of Radiation Protection Medicine, School of Military Preventive Medicine, Air Force Medical University

⁰