Studies on the effects of <sup>12</sup>C<sup>6+</sup> ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1

MA Shuang; LI Wenjian; WANG Jufang; LU Dong; LIU Jing

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Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1

更新时间：2023-03-30

- Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 2, Pages: 125-128(2009)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.中国科学院研究生院　北京　100039
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MA Shuang, LI Wenjian, WANG Jufang, et al. Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1. [J]. Journal of Radiation Research and Radiation Processing 27(2):125-128(2009)
DOI：

MA Shuang, LI Wenjian, WANG Jufang, et al. Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1. [J]. Journal of Radiation Research and Radiation Processing 27(2):125-128(2009) DOI：

摘要

为了提高生防菌BJ1的抑菌能力，优化辐照诱变的物理参数，获得生防效果更好的高效菌种，利用,12,C,6+,对生防菌BJ1进行不同剂量离子辐照处理。结果表明，采用100 Gy剂量辐照的生防菌BJ1的存活率最高，并且在0～100 Gy之间，存活率先降后升，其存活曲线呈“鞍型”；从诱变效果看，经400 Gy辐照诱变获得的生防菌BJ1的抑菌效果有明显提高；综合存活和抑菌效果考虑，离子辐照诱变生防菌BJ1的最适剂量为200～400 Gy。通过16S rDNA序列分析，将辐照诱变获得的BJ1菌株定为枯草芽孢杆菌。

Abstract

Biocontrol bacterium BJ1 was irradiated with different doses of ,12,C,6+, ion beam in order to improve the antagonistic ability, to optimize the parameters of ion beam irradiation and to obtain high effect of biocontrol strains. The results show that the survival rate of BJ1 irradiated with 100 Gy reaches the highest and the survival curve of BJ1 presents a tendency of going down first and then increasing, which takes a shape of "saddle" during 0 to100 Gy. In view of the effect of mutation induction, BJ1 irradiated with 400 Gy enhances its effect of antagonistic ability obviously. Based on the results of survival rate and the effect of antagonistic ability, an optimal treatment dose of ,12,C,6+, irradiation is 200 to 400 Gy for BJ1. And BJ1 has been identified to be Bacillus subtilin by 16S rDNA sequence cluster analysis.

关键词

离子辐照生防菌BJ1诱变菌种鉴定

Keywords

Ion beam irradiationBiocontrol bacterium-BJ1MutationIdentification

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Studies on the effects of 12C6+ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1

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摘要

Abstract

关键词

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references

Studies on the effects of ¹²C⁶⁺ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1