anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells <italic style="font-style: italic">in vitro</italic>

YANG Wei; LI Yanbo; ZHAO Jingguo; GONG Shouliang; CAO Jianping; LIU Fenju

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anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro

更新时间：2023-03-30

- anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 2, Pages: 120-124(2009)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院放射生物学教研室　苏州　215123
  2.吉林大学公共卫生学院卫生部放射生物学重点实验室　长春　130021
  3.中国人民解放军403医院整形外科　大连　116015
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YANG Wei, LI Yanbo, ZHAO Jingguo, et al. anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro. [J]. Journal of Radiation Research and Radiation Processing 27(2):120-124(2009)
DOI：

YANG Wei, LI Yanbo, ZHAO Jingguo, et al. anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro. [J]. Journal of Radiation Research and Radiation Processing 27(2):120-124(2009) DOI：

摘要

为探讨RNA干扰（RNA interference，RNAi）沉默乏氧诱导因子-1α（Hypoxia induced factor 1α，HIF-1α）和生存素（Survivin）基因联合X射线照射对乏氧人肝癌细胞SMMC-7721的体外抑瘤效应，利用双靶向HIF-1α和Survivin基因载体，采用阳离子脂质体介导法转染SMMC-7721细胞，经乏氧培养36 h后，分别以RT-PCR和Western blot法检测HIF-1α和Survivin基因的mRNA及其蛋白表达，分别以四甲基偶氮唑盐（MTT）法和流式细胞术检测细胞存活分数及细胞凋亡。结果表明，转染质粒pGenesil-Survivin-HIF的SMMC-7721细胞中，HIF-1α和Survivin基因mRNA表达水平与对照和阴性干扰组相比明显降低（,p,<,0.05），也未检测到HIF-1α和Survivin蛋白的表达。单、双干扰联合放疗组存活分数较照射组明显降低（,p,<,0.01），双干扰联合放疗组存活分数较其它组也明显降低（,p,<,0.01）。单、双干扰联合放疗组凋亡细胞百分数较对照组明显升高（,p,<,0.01），双干扰联合放疗组凋亡细胞百分数较其它组也明显升高（,p,<,0.01）。结果提示，质粒pGenesil-Survivin-HIF可有效地干扰乏氧SMMC-7721细胞HIF-1α和Survivin基因的mRNA及其蛋白表达。双干扰联合放疗组对乏氧SMMC-7721细胞的体外抑瘤效应明显优于单干扰联合放疗组。

Abstract

In order to investigate the anti-tumor effect of RNA interference silencing HIF-1α and Survivin genes combined with X-ray irradiation on hypoxic SMMC-7721 cells ,in vitro, we detected mRNA and protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells after transfection with pGenesil-Survivin-HIF carried by liposome by RT-PCR and Western blot respectively. Survival fraction and apoptosis of hypoxic SMMC-7721 cells ,in vitro, were detected by MTT and FCM assay respectively. The results show that mRNA expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells transfected with pGenesil-Survivin-HIF is significantly lower than that of control and negative interference group (,p,<,0.05). Protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells after transfection with pGenesil-Survivin-HIF can not be detected. Survival fraction of pGenesil-Survivin+5 Gy group, pGenesil-HIF+5 Gy group and pGenesil-Survivin-HIF+5 Gy group is significantly lower than that of 5 Gy group (,p,<,0.01). Survival fraction of pGenesil-Survivin-HIF+5 Gy group is also significantly lower than that of other groups (,p,<,0.01). Percentage of apoptosis of pGenesil-Survivin+5 Gy group, pGenesil-HIF+5 Gy group and pGenesil-Survivin-HIF+5 Gy group is significantly higher than that of control group (,p,<,0.01). Percentage of apoptosis fraction of pGenesil-Survivin-HIF+5 Gy group is significantly higher than that of other groups (,p,<,0.01). These results can be suggested that the pGenesil-Survivin-HIF could silence mRNA and protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells. Anti-tumor effect of pGenesil-Survivin-HIF+5 Gy group is more powerful than that of pGenesil-Survivin+5 Gy group or pGenesil-HIF+5 Gy group with hypoxic SMMC-7721 cells ,in vitro,.

关键词

RNA干扰X射线乏氧诱导因子-1α生存素

Keywords

RNA interferenceX-rayHIF-1αSurvivin

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Department of Radiobiology, School of Radiological Medicine and Public Health, Suzhou University

Brain and Nerve Laboratory, the First Affiliated Hospital, Soochow University

National Key Laboratory of Intense Pulsed Radiation Simulation and Effect, Northwest Institute of Nuclear Technology, Xi´an

Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University

School of Radiation Medicine and Public Health, Soochow University

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anti-tumor effect of silencing Hif-1α and Survivin genes combined with X-ray irradiation on hypoxic tumor cells in vitro

DOI：

摘要

Abstract

关键词

Keywords

references