Breeding of tryptophanase-producing <italic style="font-style: italic">Escherichia coli</italic> by use of N<sup>+</sup> ion beam implantation

PANG Min; YAO Jianming

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Breeding of tryptophanase-producing Escherichia coli by use of N⁺ ion beam implantation

更新时间：2023-03-30

- Breeding of tryptophanase-producing Escherichia coli by use of N⁺ ion beam implantation
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 3, Pages: 167-172(2009)
- 作者机构：
  
  1.中国科学院等离子体物理研究所　合肥　230031
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PANG Min, YAO Jianming. Breeding of tryptophanase-producing Escherichia coli by use of N⁺ ion beam implantation. [J]. Journal of Radiation Research and Radiation Processing 27(3):167-172(2009)
DOI：

PANG Min, YAO Jianming. Breeding of tryptophanase-producing Escherichia coli by use of N⁺ ion beam implantation. [J]. Journal of Radiation Research and Radiation Processing 27(3):167-172(2009) DOI：

摘要

利用了低能N,+,注入产色氨酸酶大肠杆菌,Escherichia coli,进行诱变选育。首先进行了离子注入条件及参数的探索，确立了以15 %甘油作为保护剂，N,+,注入能量10 keV，剂量13×10,14, cm,-2,的离子注入参数。反复筛选和连续突变后，获得高产色氨酸酶大肠杆菌，和出发菌株相比，其生物量和色氨酸酶活性均有所提高。经过发酵规模逐步扩大和酶促反应体系放大，菌体生物量可达8.2 ,g,ww, · L,-1,，其酶法催化合成L-色氨酸的产量高达110 g·L,-1,。该菌继代培养遗传性能稳定，可操作性强，有很好的工业化前景。

Abstract

In this paper, the mutation breeding on ,Escherichia coli, producing tryptophanase was studied after low energy N,+, ion beam implantation. Parameters in the N,+, ion beam implantation were firstly determined. It has been indicated that a high mutation rate of ,E.coli, could be obtained by N,+, implantation with 10 keV and 13×10,14, N,+,/cm,2, when glycerin at 15 % concentration used as protector. After continuous mutagenicity a high-yield tryptophanase-producing strain has been screened out and both of its biomass and enzymatic activity are higher than the previous levels respectively. The results of scale-up production show that the biomass could be reach 8.2 g,ww, ·L,-1, and 110 g L-tryptophan could be formed in the volume of 1L enzymatic reaction system. In addition, the characteristics of its stable descend ability and easy operation make it a promising strain for industrialization.

关键词

离子注入L-色氨酸大肠杆菌育种

Keywords

Ion implantationL-tryptophanEscherichia coliBreeding

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Breeding of tryptophanase-producing Escherichia coli by use of N+ ion beam implantation

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摘要

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Breeding of tryptophanase-producing Escherichia coli by use of N⁺ ion beam implantation