The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays

LE Chen; LI Haijun; CHENG Ying; MIN Rui

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The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays

更新时间：2023-03-30

- The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 3, Pages: 190-192(2009)
- 作者机构：
  
  1.第二军医大学海军医学系放射医学教研室　上海　200433
- 作者简介：
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LE Chen, LI Haijun, CHENG Ying, et al. The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays. [J]. Journal of Radiation Research and Radiation Processing 27(3):190-192(2009)
DOI：

LE Chen, LI Haijun, CHENG Ying, et al. The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays. [J]. Journal of Radiation Research and Radiation Processing 27(3):190-192(2009) DOI：

摘要

以不同剂量γ射线对大鼠进行全身照射，于照后24 h用ELISA试剂盒检测血清中MMP-2和9的浓度，底物明胶酶谱法检测血清基质金属蛋白酶的活性。结果表明，与其他各剂量组相比，5 Gy和6 Gy照射组，大鼠照射后24 h，血清MMP-2的浓度显著升高（,p,<,0.01），MMP-2的活性也随照射剂量增加而增加。而各照射剂量组血清MMP-9浓度和活性变化则无明显差别。结果显示，受照大鼠血清MMP-2浓度和活性的变化具有提示生物受照剂量的潜能。

Abstract

Rats were whole body irradiated by γ-rays with different doses. A commercial ELISA kit was used to analyze the concentration of MMP-2 and MMP-9 in rat’s serum. And Gelatin zymography electrophoresis was used to test the activity of serum MMPs at 24 h after irradiation. The results show that the amount and the activity of MMP-2 in rat’s serum increase with increment of irradiation doses. Compared with 1~4Gy exposed groups a significant rising of MMP-2 has been found in 5 Gy and 6 Gy exposed groups (,p,<,0.01). On the contrast, the amount and activity of MMP-9 in rat’s serum have a little change at 24 hours after irradiation in all of exposed groups. It can be deduced that the changes with amount and activity of MMP-2 may be used as a potential indicator of exposed dose in organisms.

关键词

γ射线基质金属蛋白酶生物剂量

Keywords

γ-rays irradiationMatrix metalloproteinaseBio-dose

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