The physiological responses to ,133,Cs and ,88,Sr stress and the characteristics of uptake and accumulation of ,133,Cs and ,88,Sr by ,Sorghum sudanense, were studied by pot experiments. The results show that under the low concentration of ,133,Cs or ,88,Sr stress, the stress-resistance of ,Sorghum sudanense, increases, the content of MDA decreases and the activities of POD and CAT increase. Under the high concentration of ,133,Cs or ,88,Sr stress, the lipid peroxidation are strengthened, the content of MDA increases and the activities of POD and CAT decrease. Along with the increment of concentration, the contents of chlorophyll decrease meanwhile the synthesis of chlorophyll in ,Sorghum sudanense, is inhibited under the stress. High concentration of ,133,Cs and ,88,Sr has toxicity to ,Sorghum sudanense, and has done the damage to the plant. However, there are no significant effects on the biomass of ,Sorghum sudanense,. Along with the increment of concentration, the contents of ,133,Cs or ,88,Sr increase in ,Sorghum sudanense, and the relationship of the contents: roots,>, the part above-ground,88,Sr,>,133,Cs.