Screening strains with high yield of inosine mutated by ions implantation

LIU Guosheng; HOU Ying; WU Feiyan; ZHAO Ting; WANG Xiuqiang; LI Zongyi

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Screening strains with high yield of inosine mutated by ions implantation

更新时间：2023-03-30

- Screening strains with high yield of inosine mutated by ions implantation
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 4, Pages: 224-228(2009)
- 作者机构：
  
  1.河南师范大学生命科学学院　新乡　453007
  2.新乡拓新生化科技有限公司　新乡　453000
  3.河南省离子束生物工程重点实验室　郑州　450052
- 作者简介：
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LIU Guosheng, HOU Ying, WU Feiyan, et al. Screening strains with high yield of inosine mutated by ions implantation. [J]. Journal of Radiation Research and Radiation Processing 27(4):224-228(2009)
DOI：

LIU Guosheng, HOU Ying, WU Feiyan, et al. Screening strains with high yield of inosine mutated by ions implantation. [J]. Journal of Radiation Research and Radiation Processing 27(4):224-228(2009) DOI：

摘要

以肌苷产生菌——枯草芽孢杆菌（,Bacillus subtilis, HSD06）为出发菌株，经不同剂量的N,+,离子束注入处理，定向选育磺胺胍抗性提高的突变株。获得磺胺胍突变株的最佳照射剂量为3×10,15, cm,-2,。从528个抗性提高的菌落中筛选获得B11和B13肌苷高产菌株，其摇瓶发酵水平肌苷产量为14.83 g/L和14.38 g/L，分别比出发菌株提高了16.3%和12.8%。

Abstract

N,+, ion implantation with different doses was used to screen sulfaguanidine resistance enhanced strains using ,Bacillus subtilis, HSD06 as an initial strain for mutation. It has been confirmed that the best implantion dose is 3×10,15, cm,-2, for resistance enhanced mutants. Two strains B11 and B13 with higher yield of inosine have been obtained from 528 mutants. Shake flask test shows that the yield of inosine reaches 14.83 g/L and 14.38 g/L with B11 and B13 respectively, which has increment by 16.3% and 12.8% respectively compared with that of initial strain.

关键词

肌苷离子束诱变枯草芽孢杆菌磺胺胍抗性突变

Keywords

InosineIon beam implantationBacillus subtilisSulfaguanidine resistanceMutation

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