ESR spin elimination research on reaction of triplet state of VK<sub>3</sub> with nucleic acid derivatives

DU Fuqiang; LIU Yuxia; TU Tiecheng; LIN Weizhen

您当前的位置：

首页 >

文章列表页 >

ESR spin elimination research on reaction of triplet state of VK₃ with nucleic acid derivatives

更新时间：2023-03-30

- ESR spin elimination research on reaction of triplet state of VK₃ with nucleic acid derivatives
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 5, Pages: 265-268(2009)
- 作者机构：
  
  1.中国科学院上海应用物理研究所　上海　201800
  2.中国科学院研究生院　北京　100049
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
DU Fuqiang, LIU Yuxia, TU Tiecheng, et al. ESR spin elimination research on reaction of triplet state of VK₃ with nucleic acid derivatives. [J]. Journal of Radiation Research and Radiation Processing 27(5):265-268(2009)
DOI：

DU Fuqiang, LIU Yuxia, TU Tiecheng, et al. ESR spin elimination research on reaction of triplet state of VK₃ with nucleic acid derivatives. [J]. Journal of Radiation Research and Radiation Processing 27(5):265-268(2009) DOI：

摘要

应用电子自旋共振（Electron spin resonance，ESR）自旋消减法，研究了内源性光敏剂2-甲基-1,4萘醌（VK,3,）的激发三重态（,3,VK,3,*,）与核酸及其组分的反应性顺序, 得到,3,VK,3,*,与四个核苷及四个单核苷酸的反应性顺序为Gua,>,Ade,>,Cyt,>,Thy，dGMP,>,dAMP,>,dCMP,>,TMP。结果与早前采用激光光解瞬态吸收光谱研究,3,VK,3,*,氧化DNA，测得的DNA及其组分阳离子自由基生成速率常数、核苷及核苷酸的氧化还原电位排序相一致。对,3,VK,3,*,与几种富含鸟嘌呤（G）的寡聚核苷酸：端粒DNA重复序列、端粒酶RNA亚基模版及其L6-P6发夹序列反应的ESR自旋消减法研究结果表明，其反应性大小与寡聚物中鸟嘌呤含量正相关，其中与端粒DNA的反应性最强。

Abstract

In order to study the reaction of triplet state of VK,3, with nucleic acid derivatives, the ESR (Electron spin resonance) spin elimination has been used with 4-oxo-TEMPO as spin trap. The reactivities of ,3,VK,3,* with nucleosides, nucleotides, and models of telomeric DNA, template of telomerase RNA and its L6-P6 region have been confirmed respectively. The results show that the tendency of reaction of ,3,VK,3,* with nucleosides is Gua,>,Ade,>,Cyt,>,Thy, meanwhile dGMP,>,dAMP,>,dCMP,>, TMP for reaction of ,3,VK,3,* with deoxy-nucleotides, and the one for reaction of ,3,VK,3,* with telomeric DNA is the highest in polynucleotides. This is highly accordant with both of their corresponding rate constants determined by laser flash photolysis and their oxidation-reduction potentials. It also shows that the reactivity of ,3,VK,3,* with polynucleotides is directly proportional to their G amount.

关键词

2-甲基-14-萘醌激发三重态电子自旋共振自旋消减法

Keywords

2-methyl-1;4-naphthoquinoneExcited triplet stateESR spin elimination

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

A TIME-RESOLVED STUDY ON THE PHOTODYNAMIC PRIMARY PROCESS OF ADE: SYNTHESIS AND PHOTOPHYSICS IN SOLUTION

LASER INDUCED REACTION OF ELECTRON TRANSFER FROM METHIONINE AND ITS DIPEPTIDES TO TRIPLET 4-NITROQUINOLINE 1-OXIDE

Related Author

No data

Related Institution

Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences

Shanghai Institute of Materia Medica, Academy Sinica

Department of Chemical Physics, University of Science and Technology of China

⁰

ESR spin elimination research on reaction of triplet state of VK3 with nucleic acid derivatives

DOI：

摘要

Abstract

关键词

Keywords

references

ESR spin elimination research on reaction of triplet state of VK₃ with nucleic acid derivatives