Inhibition of X-rays irradiation combined with thalidomide on glioma U251 cells

SUN Meiling; YU Jingping; LIU Fenju; LIU Haiyan; YANG Chen

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Inhibition of X-rays irradiation combined with thalidomide on glioma U251 cells

更新时间：2023-03-30

- Inhibition of X-rays irradiation combined with thalidomide on glioma U251 cells
- Journal of Radiation Research and Radiation Processing Vol. 27, Issue 6, Pages: 369-373(2009)
- 作者机构：
  
  1.苏州大学医学部放射医学与公共卫生学院　苏州　215123
  2.常州市第二人民医院　常州　213003
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SUN Meiling, YU Jingping, LIU Fenju, et al. Inhibition of X-rays irradiation combined with thalidomide on glioma U251 cells. [J]. Journal of Radiation Research and Radiation Processing 27(6):369-373(2009)
DOI：

SUN Meiling, YU Jingping, LIU Fenju, et al. Inhibition of X-rays irradiation combined with thalidomide on glioma U251 cells. [J]. Journal of Radiation Research and Radiation Processing 27(6):369-373(2009) DOI：

摘要

为探讨X射线联合沙利度胺对胶质瘤U251细胞的抑制作用，采用H,3,-TdR掺入法和细胞克隆形成法检测沙利度胺增强U251细胞的辐射敏感性；细胞划痕实验观察沙利度胺抑制U251细胞的浸润能力。结果表明，沙利度胺能够抑制U251细胞浸润、DNA合成和克隆形成，与X射线联用后抑制作用增强，表现出协同作用。当细胞存活率为50%时，60 μg/mL、100 μg/mL 沙利度胺的辐射增敏系数为1.18和1.51。实验证明X射线联合沙利度胺能显著提高胶质瘤U251细胞的辐射敏感性，对U251细胞具有辐射增敏作用。

Abstract

In order to investigate the inhibition effect of X-rays combined with thalidomide on glioma U251 cells, the cell scratch assay was used to detect the inhibition of thalidomide on U251 cells. And H,3,-TdR incorporation assay and colony formation were used to investigate the enhancement effect of thalidomide on the sensitivity of U251 cells to X-rays. It has been found that thalidomide has synergistic effect on therapy of glioma U251 cells if it is combined with X-rays irradiation as it can inhibit cells infiltration, DNA synthesis and colony formation. When the survival rate of glioma U251 cells is 50%, the radio-sensitization factors of 60 μg/mL and 100 μg/mL thalidomide is 1.18 and 1.51 respectively. The results reveal that thalidomide could significantly improve radio-sensitivity of glioma U251 cells.

关键词

沙利度胺胶质瘤U251细胞DNA合成辐射敏感性

Keywords

ThalidomideU251 cellDNA synthesisRadio-sensitivity

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