Synthesis of fullerene derivative C<sub>60</sub>-Lys and its radio-protection effects in AHH-1 cell

SUN Quan; HUANG Yuecheng; GAO Fu; LI Bailong; CUI Jianguo; ZHAO Fang; DONG Junrui; CAI Jianming

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Synthesis of fullerene derivative C₆₀-Lys and its radio-protection effects in AHH-1 cell

更新时间：2023-03-30

- Synthesis of fullerene derivative C₆₀-Lys and its radio-protection effects in AHH-1 cell
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 1, Pages: 37-41(2010)
- 作者机构：
  
  1.第二军医大学放射医学教研室　上海　200433
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SUN Quan, HUANG Yuecheng, GAO Fu, et al. Synthesis of fullerene derivative C₆₀-Lys and its radio-protection effects in AHH-1 cell. [J]. Journal of Radiation Research and Radiation Processing 28(1):37-41(2010)
DOI：

SUN Quan, HUANG Yuecheng, GAO Fu, et al. Synthesis of fullerene derivative C₆₀-Lys and its radio-protection effects in AHH-1 cell. [J]. Journal of Radiation Research and Radiation Processing 28(1):37-41(2010) DOI：

摘要

本研究通过富勒烯与赖氨酸直接反应，合成了一种富勒烯赖氨酸衍生物（C,60,-Lys），并通过元素分析、红外光谱、,1,H-NMR、,13,C-NMR 等对其分子结构进行了表征。细胞毒性研究发现C,60,-Lys 对人淋巴母细胞AHH-1的毒性较小，在1200 mg/L 浓度以下的细胞存活率和未用药对照组相比无明显差异。辐射生物效应研究发现400 mg/L 以上C,60,-Lys 预处理能显著提高受照细胞的存活率，并降低辐射诱发的凋亡率，其辐射保护效应具有明显的剂量依赖性。说明C,60,-Lys 预处理对受4 Gy γ射线照射的AHH-1 细胞具有一定的保护作用。

Abstract

In this study, we synthesized a fullerene derivative C60-Lys via direct reaction of fullerene(C,60,) and Lysine, whose molecular structure was identified by elemental analysis, infrared spectrum,1,H-NMR and ,13,C-NMR. It has been found that the chemical C,60,-Lys has little toxicity on human lymphoblast cell AHH-1. CCK-8 assay and AnnexinV/PI staining assay were performed to evaluate the cell survival rate and apoptosis after γ rays irradiation. The cell survival rate of C,60,-Lys treatment groups (≤1200 mg/L) shows no significant difference compared with the control group. The results show that pretreatment of cell with C,60,-Lys(≥400 mg/L) markedly increases the cell survival and decreases the cell apoptosis rate after irradiation exposure in a dose-dependant manner. These results can be suggested that C,60,-Lys has certain radioprotection effects to AHH-1 cell against radiation induced damages.

关键词

富勒烯辐射防护γ射线AHH-1 细胞细胞凋亡

Keywords

FullereneRadiation protectionγ- rayAHH-1 cellCell apoptosis

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Synthesis of fullerene derivative C60-Lys and its radio-protection effects in AHH-1 cell

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摘要

Abstract

关键词

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Synthesis of fullerene derivative C₆₀-Lys and its radio-protection effects in AHH-1 cell