Complex mutagenesis of <italic style="font-style: italic">Candida utilis</italic> by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione

SHAO Na; WEI Gongyuan; GE Xiaoguang; NIE Min

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Complex mutagenesis of Candida utilis by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione

更新时间：2023-03-30

- Complex mutagenesis of Candida utilis by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 2, Pages: 107-113(2010)
- 作者机构：
  
  1.苏州大学医学部基础医学与生物科学学院　苏州　215123
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SHAO Na, WEI Gongyuan, GE Xiaoguang, et al. Complex mutagenesis of Candida utilis by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione. [J]. Journal of Radiation Research and Radiation Processing 28(2):107-113(2010)
DOI：

SHAO Na, WEI Gongyuan, GE Xiaoguang, et al. Complex mutagenesis of Candida utilis by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione. [J]. Journal of Radiation Research and Radiation Processing 28(2):107-113(2010) DOI：

摘要

S-腺苷甲硫氨酸（S-adenosyl-L-methionine，SAM）和谷胱甘肽（Glutathione，GSH）均是生物体内重要的含硫类小分子活性物质，在医药、化妆品、保健品行业有着广泛的应用。本研究旨在通过复合诱变改善产朊假丝酵母,Candida utilis,细胞内的硫代谢途径，以实现SAM和GSH的高效联产。首先对出发菌株,C. utilis, SZU 07-01进行两轮紫外线诱变和一轮γ射线诱变，并通过选择性培养基（含1 g/L L-蛋氨酸，0.5 g/L乙硫氨酸，及2 g/L NaHSO,3,的YPD培养基）进行分离培养后，筛选获得一株可联产SAM和GSH的高效正突变菌株,C. utilis, SUGS-01。摇瓶发酵结果显示，SAM和GSH产量分别达到324.0 mg/L和220.5 mg/L，较出发菌株分别提高了131.4%和26.0%, 而且该菌株经连续传代10次后，遗传性状稳定, 具有良好的产业化应用前景。

Abstract

S-adenosyl-L-methionine (SAM) and glutathione (GSH) are both applied extensively in the fields of medicine, cosmetics and health maintenance for their biological functions multiplicity in vivo. In this paper, a complex mutagenesis was carried out to attenuate the contradiction so as to obtain a positive mutant which can produce SAM and GSH effectively. The parent strain ,Candida utilis, SZU 07-01 was treated by two turns of UV and once γ-rays irradiation for complex mutagenesis, following by cultivating and screening on the selective medium (YPD containing 1 g/L L-Met, 0.5 g/L ethionine and 2 g/L NaHSO,3,) for a positive mutant ,C. utilis, SUGS-01. The proliferation of ,C. utilis, SUGS-01 was then carried out in a flask, and the productions of S-adenosyl-L-methionine and glutathione were 324.0 mg/L and 220.5 mg/L, respectively, which were 131.4% and 26.0% higher than those of ,C. utilis, SZU 07-01. In addition,C. utilis, SUGS-01 shows high genetic stability even after 10 generations of cultivation, which indicates that the mutant ,C. utilis, SUGS-01 obtained in this study has stable capacity for high co-production of SAM and GSH, and can be used as industrial strain in the near future.

关键词

产朊假丝酵母联产发酵S-腺苷蛋氨酸谷胱甘肽复合诱变

Keywords

Candida utilisCo-productionS-adenosyl-L-methionineGlutathioneUV and γ-rays mutagenesis

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Complex mutagenesis of Candida utilis by UV and γ-rays for the co-production of S-adenosyl-L-methionine and glutathione

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摘要

Abstract

关键词

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