Effect of ionizing radiation on the differentiation of neural stem cells

LIU Ping; TU Yu

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Effect of ionizing radiation on the differentiation of neural stem cells

更新时间：2023-03-30

- Effect of ionizing radiation on the differentiation of neural stem cells
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 2, Pages: 124-127(2010)
- 作者机构：
  
  1.苏州大学医学部放射医学与公共卫生学院　苏州　215123
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LIU Ping, TU Yu. Effect of ionizing radiation on the differentiation of neural stem cells. [J]. Journal of Radiation Research and Radiation Processing 28(2):124-127(2010)
DOI：

LIU Ping, TU Yu. Effect of ionizing radiation on the differentiation of neural stem cells. [J]. Journal of Radiation Research and Radiation Processing 28(2):124-127(2010) DOI：

摘要

为了研究电离辐射对神经干细胞分化的影响，用无血清培养技术对神经干细胞进行培养，在传代后将神经干细胞分为4组，分别用0 Gy、0.5 Gy、1 Gy、2 Gy γ射线进行照射，在不撤除EGF、bFGF条件下对细胞进行培养，7 d后用免疫荧光方法对细胞进行巢蛋白（Nestin）检测。用同样的方法对神经干细胞进行照射，在培养基撤除EGF和bFGF后，用免疫荧光对神经元特异性烯醇化酶（NSE）、神经胶质纤维酸性蛋白（GFAP）进行检测。培养的神经干细胞在经一定剂量射线照射后，与空白对照组比较，nestin阳性细胞较未经照射的细胞阳性率降低；经过照射的神经干细胞分化为神经元细胞的比例较未经照射的细胞增加。结果表明，电离辐射具有诱导神经干细胞分化的作用。电离辐射使神经干细胞分化为神经元的比例增加。

Abstract

In order to investigate the effect of ionizing radiation on neural stem cells differentiation, we cultured neural stem cells of newborn rat in serum-free media containing EGF or bFGF .The neural stem cells were divided into 4 groups, which were irradiated by γ-rays with doses of 0, 0.5, 1, and 2 Gy. The irradiated cells were cultured under the same condition for 7 days, and the nestin content of neural stem cell was detected by immunofluorescence. The same method was carried out with irradiated cells in the culture medium after removing EGF, bFGF for 7 days, NSE and GFAP expression content and nestin were also detected by immunofluorescence. It has been found that the irradiated neural stem cells can express less nestin and differentiate more neurons compared to that of control group. Results show that ionizing radiation can induce the differentiation of the neural stem cells and make the neural stem cells differentiate more neuron.

关键词

神经干细胞电离辐射分化

Keywords

Neural stem cellIonizing radiationDifferentiation

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