Study on the relationship between DNA-PKcs and genomic instability &amp; hyper-radiosensitivity

YANG Kang; ZHU Jiayun; LI Junhong; DING Nan; HU Wentao; HE Jinpeng; SU Fengtao; LI Sha

您当前的位置：

首页 >

文章列表页 >

Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity

更新时间：2023-03-30

- Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 2, Pages: 114-118(2010)
- 作者机构：
  
  1.　武汉大学东湖分校生命科学与化学学院　武汉　430212
  2.中国科学院近代物理研究所　兰州　730000
  3.中国科学院研究生院　北京　100049
  4.兰州大学生命科学学院　兰州　730000
  5.兰州军区总医院放疗科　兰州　730050
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
YANG Kang, ZHU Jiayun, LI Junhong, et al. Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity. [J]. Journal of Radiation Research and Radiation Processing 28(2):114-118(2010)
DOI：

YANG Kang, ZHU Jiayun, LI Junhong, et al. Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity. [J]. Journal of Radiation Research and Radiation Processing 28(2):114-118(2010) DOI：

摘要

本研究旨在揭示DNA-PKcs（DNA损伤修复的关键酶之一）与基因组不稳定性、辐射超敏感性的关系。以人神经胶质瘤细胞系M059K（DNA-PKcs野生型）和M059J （DNA-PKcs缺陷型）为实验模型，用常规克隆形成法测定细胞存活率、cytochalasin-B（细胞松弛素B）阻断-微核法跟踪克隆形成过程中基因组不稳定性变化。实验结果表明，存在辐射超敏感性的DNA-PKcs野生型细胞M059K在0.2 Gy辐照后随着培养时间的延长基因组不稳定性与0.6 Gy辐照水平一致；不存在辐射超敏感性的DNA-PKcs缺失型细胞M059J经0.6 Gy辐照后，基因组不稳定性水平明显高于0.2 Gy辐照。这些结果提示，DNA-PKcs可能是导致辐射超敏感性的关键因素之一。

Abstract

To investigate the relationship between DNA-PKcs and genome instability & hyper-radiosensitivity, human glioma cell lines M059K and M059J, as a model expressing wild-type DNA-PKcs and a model defective in DNA- PKcs activity, were exposed to low doses of X-rays. Cells survival fractions were assessed by colony-forming assay and Cytochalasin-B micronucleus assay was employed to detect the genomic instability happening in each single irradiated colony. It has been found that as the post-incubation time increased, M059K cells expressing wild-type DNA-PKcs exhibited low-dose hyper-radiosensitivity and showed a similar genomic instability after 0.2 Gy and 0.6 Gy irradiations, but the M059J cells lacking in DNA-PKcs didn’t present low-dose hyper-radiosensitivity and showed a higher genomic instability of 0.6 Gy than that of 0.2 Gy. The results indicate that DNA-PKcs may act as one of the key factors that lead to low-dose hyper-radiosensitivity.

关键词

基因组不稳定性辐射超敏感性X射线DNA-PKcs

Keywords

Genomic instabilityHyper-radiosensitivityX-raysDNA-PKcs

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective effect of Lanzhou lily polysaccharide fragment on X-ray-induced injury of bone marrow nucleated cells in mice

Radioprotective effects of Angelica sinensis protein on mice

Comparison study on X-ray radiosensitivity in three tumor cell lines

Bibliometric analysis of DNA-PKcs subject lecture in radiobiology

Effects of carbon ion beams and X-rays on peripheral blood lymphocyte subsets in female patients with malignant tumors

Related Author

No data

Related Institution

Medical College of Northwest Minzu University, Laboratory of Environmental Ecology and Population Health in Northwest Minority Regions of Northwest Minzu University

College of Biological Science and Engineering, Fuzhou University

Laboratory of Radiation Oncology and Radiobiology, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital

Medical College of Northwest Minzu University

School of Public Health, University of South China

⁰

Study on the relationship between DNA-PKcs and genomic instability & hyper-radiosensitivity

DOI：

摘要

Abstract

关键词

Keywords

references